Skip to Content
Merck
All Photos(1)

Key Documents

More Documents

Safety Information

U8501

Sigma-Aldrich

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Type X, lyophilized powder, ≥40 units/mg protein

Synonym(s):

UTP:α-D-glucose-1-phosphate uridylyltransferase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9026-22-6
Enzyme Commission number:
2.7.7.9 (BRENDA, IUBMB)
EC Number:
232-811-2
MDL number:
MFCD00132561
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bakers yeast

Quality Level

200

type

Type X

form

lyophilized powder

specific activity

≥40 units/mg protein

composition

Protein, 30-60% modified Warburg-Christian

foreign activity

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.

Application

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
  • in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
  • to quantify Suc synthase (SUS) enzyme activity in rice
  • to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes

Biochem/physiol Actions

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized, sulfate-free powder containing citrate buffer salt

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

U8501-100UN-PW:
U8501-BULK:
U8501-1KU-PW:
U8501-PH:
U8501-1KU:
U8501-25UN-PW:
U8501-500UN:
U8501-VAR:
U8501-100UN:
U8501-25UN:
U8501-500UN-PW:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

D J Sutor et al.
Clinica chimica acta; international journal of clinical chemistry, 86(3), 329-332 (1978-06-15)
The concentration of pyrophosphate in urine has been determined using the enzyme uridine-5'-diphosphoglucose pyrophosphorylase. The technique is relatively quick and easy to perform. Reproducibility of results was good, and results from recovery experiments were excellent. The mean concentration of pyrophosphate
Himangi R Jayakar et al.
BMC microbiology, 11, 179-179 (2011-08-09)
A number of studies have revealed that Francisella tularensis (FT) suppresses innate immune responses such as chemokine/cytokine production and neutrophil recruitment in the lungs following pulmonary infection via an unidentified mechanism. The ability of FT to evade early innate immune
Leszek A Kleczkowski et al.
The Biochemical journal, 439(3), 375-379 (2011-10-14)
Plant pyrophosphorylases that are capable of producing UDP-sugars, key precursors for glycosylation reactions, include UDP-glucose pyrophosphorylases (A- and B-type), UDP-sugar pyrophosphorylase and UDP-N-acetylglucosamine pyrophosphorylase. Although not sharing significant homology at the amino acid sequence level, the proteins share a common
Yonglan Yu et al.
PloS one, 5(9), e12593-e12593 (2010-09-11)
The trehalose synthetic pathway is present in bacteria, fungi, plants and invertebrate animals, but is absent in vertebrates. This disaccharide mainly functions as a stress protectant against desiccation, heat, cold and oxidation. Genes involved in trehalose synthesis have been observed
Jesús Rodríguez-Díaz et al.
Journal of biotechnology, 154(4), 212-215 (2011-06-15)
UDP-sugars are widely used as substrates in the synthesis of oligosaccharides catalyzed by glycosyltransferases. In the present work a metabolic engineering strategy aimed to direct the carbon flux towards UDP-glucose and UDP-galactose biosynthesis was successfully applied in Lactobacillus casei. The
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service