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U7128

Sigma-Aldrich

Uricase from Arthrobacter globiformis

lyophilized powder, 15-30 units/mg protein (biuret)

Synonym(s):

Urate oxidase, Urate:O2 oxidoreductase

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About This Item

CAS Number:
9002-12-4
Enzyme Commission number:
1.7.3.3 (BRENDA, IUBMB)
EC Number:
232-655-5
MDL number:
MFCD00082126
UNSPSC Code:
12352204
PubChem Substance ID:
24900663
NACRES:
NA.54

form

lyophilized powder

specific activity

15-30 units/mg protein (biuret)

mol wt

tetramer ~32 kDa

storage temp.

−20°C

InChI

1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3

InChI key

VPXDHZMLJOJKOX-UHFFFAOYSA-N

General description

Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.

Application

Uricase from Arthrobacter globiformis has been used for coating the electrodes in - biosensor fabrication for the detection of uric acid in urine samples. It has also been used for analysing the effect of Zn, Cu, Mg, and Mn ions on uricase.

Biochem/physiol Actions

Arthrobacter globiformis is a major producer of uricase or urate oxidase in poultry manure and litter. Uricase hydrolyzes uric acid and causes ammonia volatilization in poultry manure. In the majority of mammals, uricase converts uric acid to allantoin, a soluble excretion product. Humans lack uricase due to its loss during the evolution of hominids. This increases the risk of humans towards gout, kidney stones, hypertension, and diabetes due to increased circulating levels of uric acid.Uricase is an important enzyme that plays a role in the purine metabolic pathway.
Molecular weight: 4 × ~32 KDa (tetramer)
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Zn and Cu were shown to inhibit the activity of uricase.

Unit Definition

One unit will convert 1.0 μmole of uric acid to allantoin per min at pH 9.0 at 25 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

U7128-100UN:
U7128-BULK:
U7128-VAR:
U7128-10UN:
U7128-25UN:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Uric acid: a danger signal from the RNA world that may have a role in the epidemic of obesity, metabolic syndrome, and cardiorenal disease: evolutionary considerations.
Seminars in Nephrology, 31(5), 394-399 (2011)
W K Kim et al.
Poultry science, 82(2), 223-231 (2003-03-07)
Inhibition of microbial uricase in poultry manure is critical to reduce NH3 volatilization, because hydrolysis of uric acid by microbial uricase is the first step in the production of NH3 gas in poultry manure. Three experiments were conducted to evaluate
Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.
Kanyong P, et al.
Analytical biochemistry, 428(1), 39-43 (2012)
Uric acid and evolution.
Bonifacio AL and Jesus MV
Rheumatology (Basel), 49(11), 2010-2015 (2010)
Effect of minerals on activity of microbial uricase to reduce ammonia volatilization in poultry manure.
Kim WK and Patterson PH
Poultry Science, 82(2), 223-231 (2003)
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