T9777
Trypsin-chymotrypsin inhibitor from Glycine max (soybean)
lyophilized powder
Synonym(s):
Bowman-Birk Inhibitor
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About This Item
biological source
Glycine max (soybean)
Quality Level
form
lyophilized powder
composition
Protein, 70-90% biuret
storage temp.
2-8°C
General description
Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops. Trypsin-chymotrypsin has anticarcinogenic properties.
Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.
Application
Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.
Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay. It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.
Unit Definition
One trypsin unit = ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).
Physical form
Lyophilized powder containing phosphate buffer salts, pH 7.6
Analysis Note
1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.
Other Notes
View more information on Trypsin Inhibitor.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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The Bowman‒Birk inhibitor (BBI), a protease inhibitor derived from soybeans, has been extensively studied in anti-tumor and anti-inflammation research. We recently reported that BBI has an anti-HIV-1 property in primary human macrophages. Because HSV-2 infection plays a role in facilitating
The Bowman-Birk inhibitor from soybeans as an anticarcinogenic agent
Kennedy AR
American Journal of Clinical Nutrition, 68(6), 1406S-1412S (1998)
Alfonso Clemente et al.
Molecular nutrition & food research, 54(3), 396-405 (2009-11-04)
Bowman-Birk inhibitors (BBI) from soybean and related proteins are naturally occurring protease inhibitors with potential health-promoting properties within the gastrointestinal tract. In this work, we have investigated the effects of soybean BBI proteins on HT29 colon adenocarcinoma cells, compared with
Increased separase activity and occurrence of centrosome aberrations concur with transformation of MDS
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PLoS ONE, 13(1), e0191734-e0191734 (2018)
Articles
Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.
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