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Safety Information

T8700

Sigma-Aldrich

Anti-Tyrosine Hydroxylase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution, sufficient for 10 blots

Synonym(s):

Rabbit Anti-TYH

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About This Item

MDL number:
MFCD07370531
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

usage

sufficient for 10 blots

species reactivity

mammals

technique(s)

immunohistochemistry (frozen sections): 1:1,000
indirect immunofluorescence: 1:1,000
western blot: 1:1,000 using rat brain lysates of PC-12 cells stimulated by okadaic acid

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TH(7054)
mouse ... Th(21823)
rat ... Th(25085)

General description

Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.

Immunogen

SDS-denatured rat tyrosine hydroxylase purified from pheochromocytoma.

Application

Anti-tyrosine hydroxylase antibody produced in rabbit has been used in western blot analysis and immunohistochemistry.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Biochem/physiol Actions

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

Target description

Tyrosine hydroxylase (TH) is involved in the conversion of phenylalanine to dopamine. It catalyzes the initial, rate-limiting step of the catecholamine biosynthetic pathway.

Physical form

Solution in 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

T8700-1VL-PW:
T8700-1VL:
T8700-VAR:
T8700-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK2517
SLCG2110
SLBX9058
SLBT6779
SLBQ3480V

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Cathy Cailotto et al.
PloS one, 9(1), e87785-e87785 (2014-02-04)
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Anne Grünewald et al.
Annals of neurology, 79(3), 366-378 (2015-11-26)
To determine the extent of respiratory chain abnormalities and investigate the contribution of mtDNA to the loss of respiratory chain complexes (CI-IV) in the substantia nigra (SN) of idiopathic Parkinson disease (IPD) patients at the single-neuron level. Multiple-label immunofluorescence was
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