Merck
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T3449

Sigma-Aldrich

TrypZean® Solution, 1×

recombinant, expressed in corn, sterile-filtered

Synonym(s):
Trypsin
NACRES:
NA.75

recombinant

expressed in corn

sterility

sterile-filtered

form

aqueous solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

7.70-8.30

shipped in

dry ice

storage temp.

−20°C

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This Item
C5914C5789F0926
Fetal Bovine Serum USDA approved, sterile-filtered, suitable for cell culture

Sigma-Aldrich

F0926

Fetal Bovine Serum

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

aqueous solution

form

liquid

form

solution

form

-

concentration

1 ×

concentration

1 ×

concentration

1 ×

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

pH

7.70-8.30

pH

6.9-7.1

pH

6.5-7.5

pH

-

General description

TrypZean® solution, 1× is formulated with TrypZean, recombinant bovine trypsin expressed in corn and manufactured utilizing ProdiGene′s proprietary transgenic plant protein expression system. This product is optimized for cell dissociation in both serum-free and serum-supplemented adherent cell cultures.

Application

TrypZean® Solution, 1× has been used:

  • for the suspension of pellet during DNA extraction
  • digestion of cells to single-cell suspension for pharmacotyping of 2D cells
  • as recombinant trypsin to induce cell division of Vero cells sub cultivated in IPT-AF medium

Features and Benefits

TrypZean® Solution provides the following features and benefits:
  • Animal component-free – eliminates the risk of viruses, BSE, or other potential adventitious agents
  • TrypZean is recombinant trypsin – using the same enzyme (with the same kinetics) for cell detachment means minimal protocol changes
  • Enzyme inhibition – soybean trypsin inhibitor and other inhibitors work the same with TrypZean as they do with native trypsins (weight-to-weight basis)
  • High purity – TrypZean provides increased specificity and eliminates contaminating activities found in lower purity enzymes
  • Convenience – TrypZean solution is a sterile-filtered solution formulated at the optimal concentration to dissociate adherent cell lines

Legal Information

Trypzean is a registered trademark of ProdiGene, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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