T3194

Tetanus toxin

from Clostridium tetani, cleaves synaptobrevin (VAMP-2)

• CAS Number: 676570-37-9
• MDL number: MFCD01095768
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: Tetanus toxin from Clostridium tetani,
• mol wt: 150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
• Quality Level: 100
• solubility: H₂O: soluble
• storage temp.: 2-8°C
• Gene Information: Clostridium tetani E88 ... tetX(1061100)

Description

General description

Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.

Application

Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.

Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells¹.

Biochem/physiol Actions

Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.

Tetanus toxin can cause spastic paralysis.

Preparation Note

Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.

Reconstitution

Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.

Analysis Note

Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H300 + H330,H370
• Precautionary Statements: P260 - P264 - P270 - P271 - P284 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1
• Target Organs: Central nervous system
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves