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Safety Information

T2400

Sigma-Aldrich

Tropomyosin from porcine muscle

lyophilized powder (contains Tris buffer salts)

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About This Item

CAS Number:
9067-56-5
MDL number:
MFCD00132526
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

Porcine muscle

Quality Level

200

Assay

≥70% protein basis (biuret)

form

lyophilized powder (contains Tris buffer salts)

technique(s)

electrophoresis: suitable

storage temp.

−20°C

General description

Tropomyosin is a member of the actin filament binding protein family. It is a ubiquitous protein present in animals and fungi. Tropomyosin is made up of two α-helical chains arranged as a coiled-coil. It exists over twenty isoforms, in different eukaryotic cells like skeletal, cardiac, and smooth muscles, brain, platelets, fibroblasts, platelets, and other non-muscle cells.

Application

Tropomyosin from porcine muscle has been used:

  • as a positive control in actin- α-tropomyosin (TPM1) co-sedimentation analysis and assay
  • in analyzing the anti-adhesive properties of fish tropomyosins
  • in far Western blot analysis

Biochem/physiol Actions

Tropomyosin is associated with the actin cytoskeleton and plays a role in stabilizing and regulating the interactions of actin with other actin-binding proteins. It also plays a role in muscle contraction by regulating the interactions of actin with myosin.
Tropomyosin together with troponin, regulates the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help confer structure and function to actins in the cytoskeleton and striated muscle function. Dimeric protein that assumes an α-helical coiled-coil conformation. In the absence of Ca2+, it inhibits muscle contractility by blocking the myosin binding sites on actin.

Analysis Note

SDS electrophoresis shows two major bands.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

T2400-.5MG:
T2400-BULK:
T2400-5MG:
T2400-1MG:
T2400-VAR:

Certificates of Analysis (COA)

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