T1299

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse

clone TH-2, ascites fluid

• MDL number: MFCD00213051
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: TH-2, monoclonal
• contains: 15 mM sodium azide
• species reactivity: human, monkey, guinea pig, rat, sheep, bovine, rabbit
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable using native or SDS-denatured TH.; western blot: 1:10,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.
• isotype: IgG1
• UniProt accession no.: P07101
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TH(7054) ; rat ... Th(25085)

Description

General description

Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tyrosine hydroxylase (TH) RNA undergoes alternative splicing resulting in four isoforms of TH subunit protein in humans. The four human isoforms are TH-1 (55.6 kDa), TH-2 (56 kDa), TH-3 (58.1 kDa), and TH-4 (58.5 kDa). Only humans express all four isoforms.

Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O₂ as cosubstrates, and Fe²⁺ as a cofactor.

Specificity

The antibody recognizes an epitope present in the N-terminal region (approx. aa 9-16) of both rodent and human tyrosine hydroxylase. Clone TH-2 reacts with the intact TH subunits.

Immunogen

rat tyrosine hydroxylase (TH).

Application

Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in

• western blotting
• indirect immunofluorescence
• immunostaining
• immunohistochemistry
• enzyme linked immunosorbent assay (ELISA)
• dot blot
• immunoprecipitation

Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in western blotting and immunocytochemistry.

Biochem/physiol Actions

Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable