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Safety Information

T0910

Sigma-Aldrich

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

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About This Item

CAS Number:
52500-60-4
MDL number:
MFCD00132423
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

Escherichia coli

Quality Level

200

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

specific activity

≥3 units/mg protein

mol wt

11.7 kDa

technique(s)

activity assay: suitable

solubility

water: 1.90-2.10 mg/mL, clear, colorless

Protein ID accession no.

M26133

UniProt accession no.

P0AA25

application(s)

cell analysis

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... trxA(948289)

General description

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Application

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

Biochem/physiol Actions

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.
Mediates the reduction of disulfide bonds in proteins.

Other Notes

GenBank® Accession Number M26133.

Unit Definition

One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

T0910-BULK:
T0910-1MG-PW:
T0910-1MG:
T0910-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Thioredoxin from Escherichia coli as a Role Model of Molecular Recognition, Folding, Dynamics and Function.
Vazquez DS
Protein and peptide letters, 22(9), 801-815 (2015)
Proteomic analysis of thioredoxin-targeted proteins in Escherichia coli.
Kumar JK
Proceedings of the National Academy of Sciences of the USA, 101(11), 3759-3764 (2004)
Mechanism and structure of thioredoxin reductase from Escherichia coli.
Williams CH Jr
Faseb Journal, 9(13), 1267-1276 (1995)
Ohr (organic hydroperoxide resistance protein) possesses a previously undescribed activity, lipoyl-dependent peroxidase.
Cussiol JR
The Journal of Biological Chemistry, 285(29), 21943-21950 (2010)
Jérôme Delroisse et al.
Antioxidants (Basel, Switzerland), 10(8) (2021-08-28)
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