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Key Documents

Safety Information

SRP6166

Sigma-Aldrich

HGF human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

F-TCF, HPTA, Hepatocyte growth factor, Hepatopoietin-A (HPTA), Scatter factor (SF)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

70 kDa (single chain, glycosylated)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

NCBI accession no.

P14210

UniProt accession no.

P14210

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HGF(3082)

General description

HGF (hepatocyte growth factor) is a multifunctional heterodimeric mitogen, with a relative molecular mass (Mr) of 82,000, consisting of a large α-subunit of Mr 69,000 and a small β -subunit of Mr 34,000. The pre-pro precursor protein is composed of 728 residues. HGF is produced by mesenchymal cells.
The hepatocyte growth factor (HGF) gene is located on human chromosome 7q21.1. It has 18 exons, 17 introns and spans approximately 70 kb. Active HGF is made up of a heavy chain and a light chain, connected covalently with the help of a disulfide bridge.

Biochem/physiol Actions

HGF (hepatocyte growth factor) functions as a ligand for tyrosine kinase receptor Met. Through interaction with Met receptor, it modulates mitogenesis, motility, and morphogenesis in a cell-specific manner. It is implicated in the growth and scattering of specific cell types, EMT (epithelial mesenchymal transition), formation of lumens and tubules, and angiogenesis. HGF-Met is also involved in neural induction, hepatic regeneration, wound healing, and normal fetal development. In primary culture, HGF acts as a strong mitogen for mature parenchymal hepatocytes, and is thought to be a hepatotrophic factor which induces liver regeneration post-partial hepatectomy and liver injury. In HER2-positive patients with metastatic gastric cancer, the serum level of this protein is linked with the outcome and resistance to trastuzumab therapy.
HGF exhibits mitogenic, motogenic and morphogenic effects in various tissues.

Physical form

Lyophilized in solution of 10 mM Tris-HCl, pH 7.4, with 1 M NaCl.

Preparation Note

Centrifuge the vial prior to opening.

Reconstitution

Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP6166-10UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Molecular cloning and expression of human hepatocyte growth factor.
Nakamura T, et al.
Nature, 342(6248), 440-443 (1989)
Serum level of hepatocyte growth factor is a novel marker of predicting the outcome and resistance to the treatment with trastuzumab in HER2-positive patients with metastatic gastric cancer.
Takahashi N, et al.
Oncotarget, 7(4), 925-938 (2016)
Neutralizing monoclonal antibodies to hepatocyte growth factor/scatter factor (HGF/SF) display antitumor activity in animal models.
Cao B, et al.
Proceedings of the National Academy of Sciences of the USA, 98(13) , 7443-7448 (2001)
Rajeswara R Pannem et al.
Hepatology (Baltimore, Md.), 60(3), 1066-1081 (2014-05-09)
Hepatic fibrosis is considered as a physiological wound-healing response to liver injury. The process involves several factors, such as hepatocyte growth factor (HGF), which restrains hepatic injury and facilitates reversibility of fibrotic reaction in response to an acute insult. Chronic
Rajani Kanteti et al.
PloS one, 9(9), e105919-e105919 (2014-09-16)
Malignant pleural mesothelioma (MPM) is an aggressive disease with a poor prognosis. Studies have shown that both MET and its key downstream intracellular signaling partners, PI3K and mTOR, are overexpressed in MPM. Here we determined the combinatorial therapeutic efficacy of
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