SRP6062

PP2C α human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): PP1A, PP2CA, PPM1A, Protein phosphatase 1A
• CAS Number: 362690-38-8
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• form: liquid
• mol wt: 46.6 kDa (418aa)
• packaging: pkg of 100 μg
• NCBI accession no.: P35813
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PPM1A(5494)

Description

General description

Protein phosphatase 2C-α (PP2C-α), also referred to as protein phosphatase, Mg²⁺/Mn²⁺ dependent 1A (PPM1A), is encoded by the gene mapped to human chromosome 4q12. The encoded protein belongs to the family of Ser/Thr protein phosphatases. It is characterized with a catalytic subunit and a constant regulatory subunit. PP2C-α is abundantly expressed in heart and skeletal muscle.

Biochem/physiol Actions

Protein phosphatase 2C-α (PP2C-α) is known to be a negative regulator of cell stress response pathways. Additionally, it has been reported to negatively regulate transforming growth factor-β (TGF-β) signaling pathway and cell cycle. PP2C-α has been shown to dephosphorylate cyclin-dependent kinases (CDKs) and it may be involved in cell cycle control. It modulates cell migration and invasion.

Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW

Physical form

1 mg/mL solution in 10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable