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Safety Information

SRP6057

Sigma-Aldrich

Activin A from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

FRP, FSH-releasing protein, Inhibin beta-1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

26.2 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | embryo: suitable

NCBI accession no.

P08476

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... INHBA(3624)

General description

Activin A is a member of the TGF (transforming growth factor)-β family of proteins produced by many cell types throughout development. Mouse Activin A has 100% amino acid sequence identity to human, rat, porcine, bovine and feline proteins. Recombinant mouse Activin A is a nonglycosylated homodimer, containing two 117 amino acid chains, with a total molecular weight of 26.2 kDa.

Application

Activin A has been used:
  • to induce differentiation of embryonic stem cells to pancreatic β cells
  • in the induction of bone marrow-derived mesenchymal stem cells to insulin-producing cells
  • to induce endoderm differentiation from human embryonic stem cells
  • as a component of β cell induction medium.

Biochem/physiol Actions

Activins interact with Type I and Type II serine/threonine kinases to signal to SMAD proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, metabolism, etc. Activin A is a homodimer of two β A chains and is not biologically active until the N terminal propeptide is cleaved from each chain. Activin A is involved in mesoderm formation and hematopoietic development.

Physical form

Recombinant mouse Activin A is lyophilized with 0.02% TFA.

Preparation Note

Centrifuge the vial prior to opening.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water, which can then be further diluted to other aqueous solutions.

Other Notes

MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

SRP6057-10UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Inducing embryonic stem cells to differentiate into pancreatic beta cells by a novel three-step approach with activin A and all-trans retinoic acid.
Shi Y
Stem Cells, 23, 656-662 (2005)
Functional analysis of activins during mammalian development.
Matzuk MM
Nature, 374, 354-356 (1995)
Different phenotypes for mice deficient in either activins or activin receptor type II.
Matzuk MM
Nature, 374, 356-360 (1995)
In vitro derivation of functional insulin-producing cells from human embryonic stem cells.
Jiang W
Cell Research, 17, 333-344 (2007)
Requirement for activin A and transforming growth factor--beta 1 pro-regions in homodimer assembly.
Gray AM
Science, 247, 1328-1330 (1990)
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