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Key Documents

Safety Information

SRP3287

Sigma-Aldrich

IL-34 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):

C16orf77

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

Assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

52.5 kDa

packaging

pkg of 10 μg

impurities

<0.1 EU/μg endotoxin, tested

UniProt accession no.

Q6ZMJ4

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL34(146433)

General description

IL-34 is a ligand for colony-stimulating factor-1 receptor (CSF1R), which also binds to CSF-1. It specifically binds CD14+ monocytes, promotes survival and proliferation of human peripheral blood monocytes, and stimulates macrophage colony formation by human bone marrow cells. IL-34, like human CSF-1 stimulates phosphorylation of MAPK1/ERK2 and MAPK3/ERK1.

Biochem/physiol Actions

IL-34 is a homodimeric cytokine that is expressed in a range of tissues including spleen, heart, brain, liver, kidney, lung, ovary, thymus, testis, small intestine, prostate and colon. Recombinant human IL-34 is a 52.5 kDa homodimeric glycoprotein consisting of 460 amino acid residues, including a C-terminal His-Tag.

Physical form

Lyophilized from 20 mM Sodium Citrate, pH 3.0 + 75 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319,H335

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP3287-10UG:

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Certificates of Analysis (COA)

Lot/Batch Number
0921520
219520
0618520
0716520
0515520-1

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Editorial: Leishmania survival mechanisms: the role of host phosphatases.
Marina Tiemi Shio et al.
Journal of leukocyte biology, 88(1), 1-3 (2010-07-02)
Xue Lin et al.
Critical care medicine, 46(6), e584-e590 (2018-02-01)
Sepsis is a devastating condition with a high mortality rate and limited treatments. Sepsis is characterized by a failed host immune response to contain the infection, resulting in organ dysfunction. Interleukin-34 is new cytokine involved in infection and immunity. Whether
Eleonora Franzè et al.
Journal of Crohn's & colitis, 10(1), 87-94 (2015-10-10)
Production of chemokines by intestinal epithelial cells is a key step in the amplification of the destructive immune-inflammatory response in patients with inflammatory bowel diseases [IBD]. In this study, we examined whether intestinal epithelial cells express macrophage colony-stimulating factor receptor
Eleonora Franzè et al.
Clinical science (London, England : 1979), 129(3), 271-280 (2015-03-25)
IBD (inflammatory bowel disease)-related tissue damage occurs in areas which are massively infiltrated with monocytes/macrophages. These cells respond to inflammatory stimuli with enhanced production of cytokines/chemokines. In the present study, we analysed the expression and role of IL (interleukin)-34, a
Eva Rietkötter et al.
Oncotarget, 6(17), 15482-15493 (2015-06-23)
The mononuclear phagocytic system is categorized in three major groups: monocyte-derived cells (MCs), dendritic cells and resident macrophages. During breast cancer progression the colony stimulating factor 1 (CSF-1) can reprogram MCs into tumor-promoting macrophages in the primary tumor. However, the

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