SRP2150
MKP3, GST tagged human
recombinant, expressed in E. coli, ≥85% (SDS-PAGE)
Synonym(s):
MKP3, PYST1
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.26
biological source
human
recombinant
expressed in E. coli
Assay
≥85% (SDS-PAGE)
form
frozen liquid
mol wt
~53.2 kDa
packaging
pkg of 10 μg
concentration
450 μg/mL
color
clear colorless
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... DUSP6(1848)
General description
MKP3 (MAP (mitogen-activated protein) kinase phosphatase 3), or DUSP6 (dual specificity phosphatase 6), gene is localized to human chromosome 12q22, and codes for extracellular signal-regulated kinase (ERK) specific dual-specificity phosphatase. MKP3 protein resides in the cytoplasm.
Biochem/physiol Actions
MKP3 (MAP (mitogen-activated protein) kinase phosphatase 3), or DUSP6 (dual specificity phosphatase 6), participates in one of the feedback loops regulating MAPK signal pathways in normal cell growth, where it dephosphorylates activated ERK (extracellular signal-regulated kinase) and inhibits growth-inducing signals. This protein localizes in the cytoplasm, and prevents ERK translocation to nucleus to act upon its effectors. In vitro studies in primary pancreatic cancer tissues show that this protein induces apoptosis and acts as a tumor suppressor gene. It acts as a negative feedback regulator of FGFR (fibroblast growth factor receptor) signaling, and mutations in this gene might be linked with unexplained FGFR-like syndrome cases. Expression levels of MKP3 gene in tumor samples might have potential as prognostic markers in non-small cell lung cancer (NSCLC), and the rs2279574 variant in MKP3 gene can help predict the chemoradiotherapy outcome in patients with inoperable NSCLC. In atypical endometrial hyperplasia (AEH), this gene can also help predict the effectiveness of progestin therapy.
The mitogen-activated protein kinase phosphatases (MKPs) are dual specificity phosphatases (DUSPs) that dephosphorylate phospho-Ser/Thr and phospho-Tyr residues. At least ten mammalian MPKs have been identified. MKPs have been studied for their important roles in MAPK related cancer cells and innate immune response. The MKP3, known as DUSP6 (dual specificity protein phosphatase 6), is a member of the protein tyrosine phosphatase superfamily. The MKP3 tightly regulates to ERK substrates by dephosphorylating both the phospho-Ser/Thr and phospho-Tyr residues. Its kinase interaction domain (KIM, residues 61-75) in addition to a conserved cytosolic domain (residues 161-177) and C-terminus domain (residues 348-381) plays an important role in ERK2 binding. Identification of specific activators or inhibitors of MKP3 and MPK1 may lead to the development of drug candidates against ERK pathway related diseases.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
SRP2150-10UG:
Certificates of Analysis (COA)
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Roland Lang et al.
Journal of immunology (Baltimore, Md. : 1950), 177(11), 7497-7504 (2006-11-23)
The MAPK family members p38, JNK, and ERK are all activated downstream of innate immunity's TLR to induce the production of cytokines and inflammatory mediators. However, the relative intensity and duration of the activation of different MAPK appears to determine
Robin J Dickinson et al.
Journal of cell science, 119(Pt 22), 4607-4615 (2006-11-10)
A structurally distinct subfamily of ten dual-specificity (Thr/Tyr) protein phosphatases is responsible for the regulated dephosphorylation and inactivation of mitogen-activated protein kinase (MAPK) family members in mammals. These MAPK phosphatases (MKPs) interact specifically with their substrates through a modular kinase-interaction
Prognostic value of dual-specificity phosphatase 6 expression in non-small cell lung cancer.
Diaz-Garcia CV, et al.
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 36(2), 1199-1206 (2015)
Dusp6 (Mkp3) is a negative feedback regulator of FGF-stimulated ERK signaling during mouse development.
Li C, et al.
Development, 134(1), 167-176 (2007)
Dual Specificity Phosphatase 6 (DUSP6) Polymorphism Predicts Prognosis of Inoperable Non-Small Cell Lung Cancer after Chemoradiotherapy.
Wang TL, et al.
Clinical Laboratory, 62(3), 301-310 (2016)
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