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Key Documents

Safety Information

SRP2082

Sigma-Aldrich

WT-1 (+KTS) human

recombinant, expressed in insect cells, ≥60% (SDS-PAGE)

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.26

biological source

human

recombinant

expressed in insect cells

Assay

≥60% (SDS-PAGE)

form

frozen liquid

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

150 μg/mL

color

clear colorless

NCBI accession no.

NM_024425

UniProt accession no.

P19544

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... WT1(7490)

Biochem/physiol Actions

WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. With the inclusion of exon 5, WT1 (KTS+) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also co-localizes with splicing factors in a speckled nuclear particle, suggesting that the WT1 protein may function as both a transcription factor and a splicing regulator.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

SRP2082-5UG:

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Certificates of Analysis (COA)

Lot/Batch Number
093071073

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J A Kreidberg et al.
Cell, 74(4), 679-691 (1993-08-27)
In humans, germline mutations of the WT-1 tumor suppressor gene are associated with both Wilms' tumors and urogenital malformations. To develop a model system for the molecular analysis of urogenital development, we introduced a mutation into the murine WT-1 tumor
D A Haber et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(21), 9618-9622 (1991-11-01)
The chromosome 11p13 Wilms tumor susceptibility gene WT1 appears to play a crucial role in regulating the proliferation and differentiation of nephroblasts and gonadal tissue. The WT1 gene consists of 10 exons, encoding a complex pattern of mRNA species: four
S H Larsson et al.
Cell, 81(3), 391-401 (1995-05-05)
WT1 is a tumor suppressor gene with a key role in urogenital development and the pathogenesis of Wilms' tumor. Two alternative splice sites in the WT1 transcript allow the gene to encode four proteins. These carry four Krüppel-type zinc fingers

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