Skip to Content
Merck
All Photos(2)

Key Documents

Safety Information

SRP0734

Sigma-Aldrich

PIP4K2B, Active

PRECISIO® Kinase, recombinant

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.27

recombinant

expressed in baculovirus infected Sf9 cells

Assay

70-95% (SDS-PAGE)

form

frozen liquid

specific activity

4500-6000 nmol/min·mg (ADP-Glo)

mol wt

~73 kDa

concentration

0.1 μg/μL

accession no.

NM_003559

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PIP4K2B(8396)

Application

Useful for kinase assay.

Biochem/physiol Actions

Recombinant full-length human PIP4K2B was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP4K2B (phosphatidylinositol-5-phosphate 4-kinase, type II, beta) catalyzes the phosphorylation of phospha-tidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. PIP4K2B is a member of the phosphatidylinositol-5-phosphate 4-kinase family. PIP5K2B interacts specifically with the juxtamembrane region of the p55 TNF receptor and that PIP5K2B activity is increased in mammalian cells by treatment with TNF-alpha (1). PIP5P4KB plays a role in determining insulin sensitivity and adiposity (2).

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP0734-10UG:

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
W3136-4

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service