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Key Documents

Safety Information

SRP0181

Sigma-Aldrich

AHCY Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

Adenosylhomocysteinase, AdoHcyase, CuBP, S-Adenosyl-L-homocysteine hydrolase, SAHH

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

73 kDa

packaging

pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

NM_000687

UniProt accession no.

P23526

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... AHCY(191)

General description

Human AHCY (human S-adenosyl homocysteine hydrolase) (GenBank Accession No. NM_000687), (amino acids 2-end) with N-terminal GST tag, MW = 73 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP0181-50UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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