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Safety Information

SML2959

Sigma-Aldrich

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Ready Made Solution

≥98% (HPLC), liquid, mithophagy inducer

Synonym(s):

FCCP, Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone

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About This Item

Empirical Formula (Hill Notation):
C10H5F3N4O
Molecular Weight:
254.17
UNSPSC Code:
12352200
NACRES:
NA.77

Product Name

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Ready Made Solution, ≥98% (HPLC), 2.54 mg/mL (10mM) in DMSO

Quality Level

100

Assay

≥98% (HPLC)

form

liquid

storage temp.

−20°C

General description

Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) is a proton (H+) ionophore and a potent mitochondrial uncoupling agent. This compound turns the mitochondrial membrane permeable to protons, thus dissipating the mitochondrial membrane potential and uncouples oxidative phosphorylation from ATP synthesis. FCCP is a potent tool for assaying mitochondrial respiration and investigating the role of mitochondria in cellular function.1,2FCCP induces mitophagy of SH-SY5Y neuroblastoma cell in a dose dependent manner. Low concentration of FCCP (1μM) leads to a complete loss of mitochondrial membrane potential without triggering mitophagy, whereas at a high concentration (10 μM), cytosol acidification is increased and mitochondrial degradation is attained.3 Additionally, FCCP (20 μM) was able to induce apoptosis in lymphoid (CCRF-CEM) and myeloid (HL60) cells after 24hrs of incubation. 4 FCCP (0.5nM-5 μM) reduces β-amyloid peptide production in K695sw cells without altering the β-amyloid precursor protein maturation or synthesis.5 Partial uncoupling of mitochondrial oxidative phosphorylation induced by FCCP (0.3 -3μM) reduces micromotion and wound healing migration of human mesenchymal stem cells.6 Inhibition of F1F0-ATPase by FCCP (10 μM), reduces the impairment of electromechanical activity in human myocardium.7

Biochem/physiol Actions

Mithophagy inducer, ionophore

Other Notes

FCCP ready made solution is a DMSO based solution at 10mM concentration. It is recommended to further dilute in a suitable media to achieve a working concentration of 1-10 μM.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

FSL

Group 4: Flammable liquids
Type 3 petroleums
Hazardous rank III
Water insoluble liquid

JAN Code

SML2959-BULK:
SML2959-1ML:4548173368047
SML2959-1ML-PW:
SML2959-VAR:

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