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Key Documents

Safety Information

SML1154

Sigma-Aldrich

Clostridium difficile Toxin A

≥95% (SDS-PAGE), lyophilized powder, cellular toxin

Synonym(s):

TcdA

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About This Item

MDL number:
MFCD02098001
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Clostridium difficile Toxin A, ≥95% (SDS-PAGE)

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

storage temp.

2-8°C

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General description

Native toxin A purified from C. difficile strain VPI10463 (toxinotype 0)

Biochem/physiol Actions

Clostridium difficile Toxin A and B, cation-dependent UDP-glucose glucosyltransferases, are cellular toxins that inactivate Rho (and Rho family small GTPases) through monoglucosylation of these family members. TcdA elicits effects primarily within the intestinal epithelium. Effects of this monoglucosylation include disregulation of the actin cytoskeleton, cell rounding, cytotoxicity, and altered cellular signaling. Rho proteins are monoglucosylated by Toxin A and B using UDP-glucose as a cosubstrate. Rho, Rac and Cdc42 are included in the Rho subfamilies targeted by both toxins. Low molecular mass GTP-binding proteins that are not modified by Toxin A and B include Ras, Rab, Arf, or Ran subfamilies as well as heterotrimeric G proteins.

Reconstitution

Contents of the 50UG sku when reconstituted in 250 μL sterile distilled water will contain Toxin A at a concentration of approximately 0.2 mg/mL in 0.05M Hepes, 0.15M sodium chloride and 5% sucrose.
Contents of the 2UG sku when reconstituted in 250 μL sterile distilled water will contain Toxin A at a concentration of approximately 8 μg/mL in 0.05M Hepes, 0.15M sodium chloride and 5% sucrose.

Other Notes

After reconstitution, the reconstituted liquid can be frozen at -80 °C to -20 °C to extend stability to 1 year; however every freeze/thaw cycle wil cause increased aggregation. Avoid multiple freeze/thaw cycles and multiple temperature fluctuations.
Mixing: Swirl or pipette gently to mix. Vortexing may promote aggregation.

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H301,H312 + H332

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SML1154-2UG:
SML1154-50UG:
SML1154-VAR:
SML1154-50UG-LBL:
SML1154-BULK:
SML1154-2UG-LBL:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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