Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

SCP0158

Sigma-Aldrich

Granzyme B Substrate

≥95% (HPLC), lyophilized

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C32H41N5O11
Molecular Weight:
671.69
UNSPSC Code:
12352204
NACRES:
NA.32

product name

Granzyme B Substrate,

Assay

≥95% (HPLC)

form

lyophilized

composition

Peptide Content, ≥85%

storage condition

protect from light

storage temp.

−20°C

Amino Acid Sequence

Ac-Ile-Glu-Pro-Asp-AMC

Application

Granzyme B Substrate (Ac-IEPD-AMC) is a fluorogenic substrate for the detection and assay of caspase 8 and granzyme B which is involved in the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Biochem/physiol Actions

Granzymes belongs to serine proteinase family enzymes and it localizes along with perforin in granules. It is predominant in cytotoxic T cells. Granzyme B induces caspases dependent and independent cell death. It possesses specific cleavage action at aspartate residue in the substrate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCP0158-5MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Fundamental Immunology, 1083-1083 (2008)
Basic Concepts of Molecular Pathology, 32-32 (2009)
M Hayashida et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 225(2), 143-150 (2000-10-24)
Cell death induction by cytotoxic T lymphocytes (CTLs) is an important thesis for the understanding of tumor immunotherapy. In the current study we investigated the molecular machinery of CTL-induced cell death in human hepatocellular carcinoma cell lines (HCC lines). CTLs
Hideaki Toda et al.
Veterinary immunology and immunopathology, 141(1-2), 168-172 (2011-03-26)
Granzyme B plays an important role in granule-mediated apoptosis by CTL. It is a well characterized component of the cytolytic machinery in mammals and a candidate for the evaluation of cytotoxic activity of CTL as an alternative to conventional cytotoxicity
Marcelo Marcet-Palacios et al.
PLoS pathogens, 7(12), e1002447-e1002447 (2011-12-24)
Cytotoxic T lymphocytes (CTLs) are the major killer of virus-infected cells. Granzyme B (GrB) from CTLs induces apoptosis in target cells by cleavage and activation of substrates like caspase-3 and Bid. However, while undergoing apoptosis, cells are still capable of
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service