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Safety Information

SAE0198

Sigma-Aldrich

Calprotectin, human recombinant

Stabilized heterodimer of fusion tagged S100A9 and S100A8

Synonym(s):

MRP8/14 and S100A8/A9 complex

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

shipped in

wet ice

storage temp.

−20°C

General description

This recombinant human calprotectin is a heterodimer composed of tagged versions of both S100A8 and S100A9, where a 6xHis-SUMO tag is added to the N-terminus of S100A9, and a 6xHIS tag is added to the N-terminus of S100A8. This tagged version was found to have enhanced stability as compared to untagged versions of this protein. Removal of the tags can be achieved using HRV-3C protease (SAE0045 or SAE0110).

Application

Fecal Calprotectin is often used as a biomarker to identify inflammation in gastrointestinal disease1. This product is a recombinant version of the human protein found endogenously.

Biochem/physiol Actions

Calprotectin is a calcium and zinc binding protein that belongs to the S100 family. It is a heterodimer protein composed of S100A8 and S100A9.1 The protein is expressed in myeloid cells and in circulating monocytes and neutrophils, where it accounts for 60% of the cytosolic protein content. Calprotectin was found to play major roles in inflammation and the immune response by activation of NF-kB pathway via TLR4 and AGER,2 and its antimicrobial activity possibly through chelating of Zn.3 High concentrations of calprotectin are found and serve as a biomarker in rheumatoid, cystic fibrosis, inflammatory bowel disease and in Crohn′s disease.4 A meta-analysis also identified calprotectin as a potential biomarker for COVID-19.5

Physical form

The product is lyophilized from a sterile 0.2 µm filtered solution containing 12.5 mM Tris-Cl (pH 8.0), 150 mM NaCl, and 1 mM DTT.

Preparation Note

Reconstitute lyophilized vial in DMEM or in PBS to a concentration up to 500 µg/mL. Spin down to collect material. Reconstituted product should be stored in working aliquots at –20 °C. Do not store in a frost-free freezer. Avoid freeze-thaw cycles.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAE0198-VAR:
SAE0198-BULK:
SAE0198-50UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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