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Safety Information

SAE0116

Sigma-Aldrich

Heparanase-1 pre-activated human

recombinant, expressed in HEK 293 cells

Synonym(s):

Endo-glucoronidase, HPA, HPA1, HPR1, HPSE, HPSE1, HSE1, Heparanase

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About This Item

Enzyme Commission number:
3.2.1.166 (BRENDA, IUBMB)
UNSPSC Code:
12352202
NACRES:
NA.26

recombinant

expressed in HEK 293 cells

Assay

≥95.0% (SDS-PAGE)

form

lyophilized powder

impurities

≤1.00 EU/μg (LAL)

UniProt accession no.

Q9Y251

shipped in

ambient

storage temp.

−20°C

Related Categories

General description

Recombinant human heparanase-1 is expressed in human HEK 293 cells as an active heterodimer glycoprotein containing a 50 kDa subunit and a 8 kDa subunit. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.

Biochem/physiol Actions

Heparanase-1 (heparanase) is the only heparan sulfate degrading endoglycosidase. It is involved in the regulation of multiple biological processes that increase tumor growth, metastasis, angiogenesis and inflammation. Heparanase is involved in ECM remodeling and heparan sulfate-linked biological molecules release, including cytokines and growth factors. Heparanase cleaves the heparan sulfate side chains of heparan sulfate proteoglycans into fragments of 10–20 sugar units.
Heparanase is initially translated as a preproenzyme containing a signal peptide. Cleavage of the signal sequence yields a latent 65 kDa pro-heparanase, which must undergo further processing for activity. Mature active heparanase is a heterodimer consisting an N-terminal 8 kDa subunit and a C-terminal 50 kDa subunit.

Unit Definition

One heparanase activity unit is the amount of heparanase required to cleave 1 pmol of pentasaccharide to disaccharide and trisaccharide in one minute, at 37 °C, pH 5.0.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAE0116-10UG:
SAE0116-VAR:
SAE0116-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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