Skip to Content
Merck
All Photos(1)

Documents

Safety Information

SAE0104

Sigma-Aldrich

BmrA (Bacilus Subtilis Multidrug Resistance Abc Transporter Atp-Binding Protein)

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.83

recombinant

expressed in E. coli

description

C-Terminal six-histidine tagged

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

64.5 kDa

concentration

0.3 mg/mL

UniProt accession no.

O06967

shipped in

dry ice

storage temp.

−70°C

Biochem/physiol Actions

BmrA is an efflux protein and ABC transporter that acts as an ATP-dependent drug efflux pump.

Preparation Note

Formulated in 50mM Tris-Cl pH 8.0, 100mM NaCl, 0.01% DDM, 10% glycerol.

Storage and Stability

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot into single use aliquots. Store remaining undiluted protein in aliquots at -80°C. Note: avoid freeze/thaw cycles.

Substrates

MSSSHHHHHHMPTKKQKSKSKLKPFFALVRRTNPSYGKLAFALALSVVTTLVSLLIPLLTKQLVDGFSMSNLSGTQIGLIALVFFVQAGLSAYATYALNYNGQKIISGLRELLWKKLIKLPVSYFDTNASGETVSRVTNDTMVVKELITTHISGFITGIISVIGSLTILFIMNWKLTLLVLVVVPLAALILVPIGRKMFSISRETQDETARFTGLLNQILPEIRLVKASNAEDVEYGRGKMGISSLFKLGVREAKVQSLVGPLISLVLMAALVAVIGYGGMQVSSGELTAGALVAFILYLFQIIMPMGQITTFFTQLQKSIGATERMIEILAEEEEDTVTGKQIENAHLPIQLDRVSFGYKPDQLILKEVSAVIEAGKVTAIVGPSGGGKTTLFKLLERFYSPTAGTIRLGDEPVDTYSLESWREHIGYVSQESPLMSGTIRENICYGLERDVTDAEIEKAAEMAYALNFIKELPNQFDTEVGERGIMLSGGQRQRIAIARALLRNPSILMLDEATSSLDSQSEKSVQQALEVLMEGRTTIVIAHRLSTVVDADQLLFVEKGEITGRGTHHELMASHGLYRDFAEQQLKMNADLENKAG

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAE0104-10UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Emmanuelle Steinfels et al.
Biochemistry, 43(23), 7491-7502 (2004-06-09)
The involvement of transporters in multidrug resistance of bacteria is an increasingly challenging problem, and most of the pumps identified so far use the protonmotive gradient as the energy source. A new member of the ATP-binding cassette (ABC) family, known

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service