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Safety Information

SAE0003

Sigma-Aldrich

4-Hydroxyphenylpyruvate Dioxygenase from Streptomyces avermitilis

recombinant, expressed in E. coli

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About This Item

Enzyme Commission number:
1.13.11.27 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

form

liquid

specific activity

≥5 U/mg

mol wt

~41.8 kDa

shipped in

dry ice

storage temp.

−70°C

General description

4-Hydroxyphenylpyruvate Dioxygenase (4-HPPD) is a key enzyme in the catabolism of tyrosine and phenylalanine. 4-HPPD catalyzes the synthesis of homogenistate (2,5-dihydroxyphenylacetate) from ρ-hydroxyphenylpyruvate and molecular oxygen. 4-HPPD also catalyzes the conversion of α-ketoisocaproate to Β-hydroxyisovalerate. 4-HPPD requires Fe2+ as an essential cofactor.

Rat 4-HPPD has a minimum of 80% homology with human 4-HPPD. Rat 4-HPPD is expressed abundantly in hepatocytes. It is also expressed in kidney, particularly in epithelial cells of the proximal tubules and the distant tubules. 4-HPPD has been identified as a potential biomarker of drug-induced liver injury in rat studies.

Unit Definition

One unit will oxidize 1 μmole of molecular oxygen per minute at pH 7.0, 25 °C, in the presence of 4-hydroxyphenylpyruvate.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAE0003-1MG-PW:
SAE0003-VAR:
SAE0003-1MG:
SAE0003-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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