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Safety Information

SAB5900208

Sigma-Aldrich

Alpaca Anti-mCherry, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

Synonym(s):

MCherry fluorescent protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

alpaca

Quality Level

recombinant

expressed in E. coli

conjugate

unconjugated

antibody form

affinity purified VHH antibody

clone

RP55, monoclonal

Assay

>95% (SDS-PAGE)

form

lyophilized solid

mol wt

14.3 kDa
~15-18 kDa (SDS PAGE under reducing condition)

purified by

immobilized metal affinity chromatography (IMAC)

concentration

1 mg/mL

technique(s)

ELISA: 1:2000-1:10000
immunofluorescence: 1:500-1:2000
western blot: 1:1000-1:5000

color

colorless

pH

( in PBS, pH 7.4)

isotype

VHH

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy. mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel. Alpaca derived anti-mCherry VHH single domain antibody (molecular weight: 14.3 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

Specificity

mCherry

Immunogen

Recombinant full length mCherry protein

Application

ELISA, WB, IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.11

Reconstitution

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 year at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAB5900208-50UG:
SAB5900208-100UG:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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