SAB5900205

Alpaca Anti-Her2, recombinant single domain Antibody

Recombinant Alpaca (VHH) Nano antibody

• Synonym(s): CD340, HER-2, HER2, c-ERB2, p185(erbB2)
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: alpaca
• Quality Level: 100
• recombinant: expressed in E. coli
• conjugate: unconjugated
• antibody form: affinity purified VHH antibody
• clone: EB02, monoclonal
• Assay: >95% (SDS-PAGE)
• form: lyophilized solid
• mol wt: 13.7 kDa; ~15-18 kDa
• purified by: immobilized metal affinity chromatography (IMAC)
• species reactivity: human
• concentration: 1 mg/mL
• technique(s): immunofluorescence: 1:200-1:600; immunohistochemistry: 1:200-1:600
• color: colorless
• pH: ( in PBS, pH 7.4)
• isotype: VHH
• Protein ID accession no.: P04626.1
• UniProt accession no.: P04626
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ERBB2(2064)

Description

General description

Receptor tyrosine-protein kinase erbB-2, also known as Her2, is a protein that in humans is encoded by the ERBB2 gene. The ErbB family consists of four closely related tyrosine kinase receptors that act as potent mediators of normal cell growth and development. Aberrant expression or function of one or more of these receptors can play a major role in the development and evolution of cancer. Her2 has been implicated in the evolution of both breast and gastric cancers, and is evident in other cancer types such as ovarian and salivary gland tumors. Her2 possesses an active tyrosine kinase domain, but no direct ligand has been identified yet. Her2 is the preferred binding partner to the other members of the ErbB family and is thought to act primarily through the Ras-MAPK, PI3k-PKB/Akt, and PLC-PKC signalling pathways.Alpaca derived anti-HER2 VHH single domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

Specificity

Human Epidermal Growth Factor Receptor 2 (Her2)

Immunogen

Recombinant human HER2 (Thr23-Thr652) protein

Application

IHC,IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.8

Reconstitution

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 year at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable