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Safety Information

SAB5201915

Sigma-Aldrich

Monoclonal Anti-ERp57-Atto 390 antibody produced in mouse

clone 4F9, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-PDIA3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.45

biological source

mouse

conjugate

Atto 390 conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

4F9
monoclonal

description

target molecular weight: 57 kDa

form

buffered aqueous solution

species reactivity

human (Tested)

packaging

pkg of 100 μg

concentration

1.0 mg/mL

technique(s)

immunofluorescence: 1:100
western blot: 1:1000

isotype

IgG2b

Protein ID accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PDIA3(2923)

Immunogen

Immunogen type: Recombinant protein
Immunogen Species: Human
Immunogen Region: See Notes section/Other
Immunogen Description: Human recombinant ERp57 (Grp58) protein corresponding to amino acids 25 - 505 (his-tagged)

Application


Western blotting
Dilution 1:1000
Tissue/Cell Type Used: HeLa cell lysate

Immunofluorescence
Dilution 1:100
Tissue/Cell Type Used: HeLa

Target description

ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface . ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus .

Physical form

50% glycerol, 0.09% Sodium Azide
PBS pH 7.4

Preparation Note

Derived from cell/tissue culture

Storage and Stability

Protect from light; avoid repeated freeze-thaw cycles. Store at -20 °C long-term, 2-8 °C short-term.

Other Notes

Additional Product Details:
Immunogen region: Amino acids 25-505
Lot Expiration/Retest Date: Expires 1 year from purchase

Other:
Immunogen region: Amino acids 25-505
Purification method: Protein G purified
Product type: Primary antibody
Antibody structure: Whole Antibody
Protein ID: NP_005304.3

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAB5201915-100UG:


Certificates of Analysis (COA)

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