SAB4702062

Monoclonal Anti-HLA-E purified produced in mouse

• Synonym(s): HLA class I histocompatibility antigen, MHC class I antigen E, alpha chain E
• UNSPSC Code: 12352203

Properties

• conjugate: unconjugated
• Quality Level: 100
• antibody product type: primary antibodies
• clone: MEM-E/02, monoclonal
• form: buffered aqueous solution
• species reactivity: ( Non-human primates), human
• concentration: 1 mg/ml
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: P13747
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... HLA-E(3133)

Description

General description

HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).

Specificity

The antibody MEM-E/02 specifically reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.

Immunogen

Recombinant human HLA-E denaturated heavy chain.

Physical form

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified by protein-A affinity chromatography.

Storage and Stability

Store at 2-8°C. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable