SAB4200748

Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse

clone MB-11, purified immunoglobulin

• Synonym(s): Anti-Human immunoglobulin M
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MB-11, monoclonal
• form: lyophilized powder
• species reactivity: human
• concentration: ~2.0 mg/mL
• technique(s): ELISA: 1:40,000-1:80,000 using 2.5 μg/mL Human IgM for coating
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgM protein. Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocytes membranes. IgM has a pentameric structure, in which monomers are linked together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells.

Specificity

Monoclonal Anti-Human IgM (μ-chain) specifically recognizes μ chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, kappa, lambda and gamma chains.

Immunogen

purified human IgM protein

Application

Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse may suitable for use in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

IgM is suggested to act as the first line of defense during microbial infections. Secreted IgM (sIgM) is involved in B cell maturation and complement activation. Detection of IgM antibodies in a patient′s serum may indicates a recent infection and IgM in a neonate′s serum may indicate on intrauterine infection (e.g. congenital rubella syndrome). In addition, the development of anti-donor IgM after combined liver-kidney transplantation has been shown to provide a graft- protecting effect. Anti-Human IgM (μ-specific) antibodies can be used in detection of the abnormal concentration of IgM in patient sera and IgM-associated pathologies, such as selective IgM immunodeficiency (SIgMD) and the hyperimmunoglobulin M (hyper-IgM or HIGM) syndromes.

Physical form

Supplied as a lyophilized powder

Storage and Stability

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable