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Safety Information

SAB4200732

Sigma-Aldrich

Anti-β-Tubulin I antibody, Mouse monoclonal

clone SAP.4G5, purified from hybridoma cell culture

Synonym(s):

Anti-TUBB

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SAP.4G5, monoclonal

form

buffered aqueous solution

mol wt

~55 kDa

species reactivity

bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.06-0.12 μg/mL using HeLa cell extract
immunofluorescence: 1-2.5 μg/mL using HeLa cells
immunohistochemistry: 1-2 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human fallopian tube or testis sections.

isotype

IgG1

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB(203068)

General description

Monoclonal Anti-β-Tubulin I (mouse IgG1 isotype) is derived from the SAP.4G5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse. Tubulin is the major building block of microtubules, an intracellular cylindrical filamentous structure, that is present in almost all eukaryotic cells. Tubulin exists in all cells as a 100 kDa protein, which assembles from heterodimers of α-tubulin and β-tubulin; both have a molecular weight of 55 kDa and share considerable homology.

Immunogen

synthetic peptide corresponding to the C-terminal sequence of ß-tubulin isotype I, conjugated to BSA

Application

Anti-β-Tubulin I antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

β-Tubulin I is also useful in deducing the role that different tubulin isotypes play in nerve development, function or maintenance.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAB4200732-100UL:
SAB4200732-BULK:
SAB4200732-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Brian Perry et al.
Journal of the Association for Research in Otolaryngology : JARO, 4(3), 329-338 (2003-12-24)
The seven mammalian isotypes of beta tubulin are strikingly similar in amino acid sequence. The differences in isotypic sequence, although small, are nonetheless conserved in evolution, which suggests that they may confer distinct functional roles. If so, such roles should
Tubulin modifications and their cellular functions
Hammond JW, et al.
Current Opinion in Cell Biology, 20(1), 71-76 (2008)
S S Siddiqui et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 9(8), 2963-2972 (1989-08-01)
We have identified 2 anti-tubulin monoclonal antibodies that bind to 2 different subpopulations of identified neurons in the simple nervous system of the nematode Caenorhabditis elegans. The antibodies also recognize specific tubulin isotypes from C. elegans that were separated by
M C Roach et al.
Cell motility and the cytoskeleton, 39(4), 273-285 (1998-05-15)
Tubulin, the subunit protein of microtubules, is an alpha/beta heterodimer. In many organisms, both alpha and beta consist of various isotypes. Although the isotypes differ in their tissue distributions, the question of whether the isotypes perform different functions in vivo

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