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Safety Information

SAB4200559

Sigma-Aldrich

Anti-VAChT (C-terminal) antibody produced in rabbit

IgG fraction of antiserum

Synonym(s):

Anti-SLC18A3, Anti-Solute carrier family 18 member 3, Anti-VAT, Anti-vesicular acetylcholine transporter

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, mouse, human

technique(s)

indirect immunofluorescence: 1:1,000-1:2,000 using PC12 cells treated with rat NGF.
western blot: 1:500-1:800 using extracts of mouse brain (S1 fraction).

UniProt accession no.

Q16572

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SLC18A3(6572)
mouse ... slc18a3(20508)
rat ... slc18a3(60422)

General description

Vesicular Acetylcholine Transporter (VAChT, SLC18A3) belongs to the solute carrier family 18 (SLC18) family of proton/neurotransmitter antiporters and shares a high degree of homology to vesicular monoamine transporters (VMATs). It is a 12-transmembrane domain protein. VAChT is expressed in all major cholinergic neurons in the central and peripheral nervous system and is mainly localized in small synaptic vesicles in cholinergic nerve terminals.

Immunogen

synthetic peptide corresponding to a sequence at the C-terminus of human vesicular acetylcholine transporter (VAChT), conjugated to KLH. The corresponding sequence is highly conserved in rat and mouse VAChT (82% identity).

Application

Anti-VAChT (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence staining
  • immunocytochemistry
  • double immunolabeling

Biochem/physiol Actions

Vesicular Acetylcholine Transporter (VAChT), is required in cholinergic neurons for the selective transport of acetylcholine (ACh) into synaptic vesicles. It depends on the activity of a vacuolar-type H+ -ATPase to accumulate ACh in synaptic vesicles. Knockdown of VAChT in the hippocampus has been shown to cause behavior changes, learning deficits, specific deficits in spatial memory and long-term potentiation (LTP). VAChT provides a specific marker for cholinergic neurons, for the study of cholinergic transmission in experimental models of Alzheimer′s disease and other nervous system disorders. Cloning of the VAChT gene has revealed a single genetic locus encoding both VAChT and ChAT, providing a unique genomic arrangement suggesting that these genes may be co-regulated.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SAB4200559-200UL:
SAB4200559-BULK:
SAB4200559-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elimination of the vesicular acetylcholine transporter in the forebrain causes hyperactivity and deficits in spatial memory and long-term potentiation
Martyn AC, et al.
Proceedings of the National Academy of Sciences of the USA, 109(43), 17651-17656 (2012)
Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons
Shi Y, et al.
Nature Medicine, 24(3), 313-313 (2018)
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Ran Lu et al.
Signal transduction and targeted therapy, 2, 16036-16036 (2017-12-22)
Recent evidences show that nervous system acts as a crucial part of cancer microenvironment. Infiltration of nerve fibers into cancer microenvironment has an important active role in cancer progression. The stimulations of both cancer growth and metastasis by members of
Alzheimer's disease: targeting the cholinergic system
Ferreira-Vieira TH, et al.
Current Neuropharmacology, 14(1), 101-115 (2016)
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