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Safety Information

S9446

Sigma-Aldrich

Sphingosine Kinase 1 human

aqueous glycerol solution

Synonym(s):

SPHK 1

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About This Item

Enzyme Commission number:
2.7.1.91 (BRENDA, IUBMB)
MDL number:
MFCD09265456
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in baculovirus infected insect cells

Quality Level

200

form

solution

specific activity

≥10 units/μg

mol wt

47 kDa

UniProt accession no.

Q9NYA1

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... SPHK1(8877)

General description

Sphingosine 1-phosphate-induced release of TIMP-2 from vascular smooth muscle cells inhibits angiogenesis.

Application

Sphingosine kinase 1 has been used in a study to assess the modulation of cellular S1P levels. Sphingosine kinase 1 has also been used in a study to investigate how FTY720 normalizes hyperglycemia.

Biochem/physiol Actions

Converts sphingosine to sphingosine-1-phosphate. SPHK1 shares two large conserved regions with SPHK2. While SPHK1 is found only in the cytosol, SPHK2 is localized in both the cytosol and nuclei.

Unit Definition

One unit will produce 1.0 pmole of sphingosine-1-phosphate from ATP and D-erythro-sphingosine per minute at pH 7.4, 37 °C.

Physical form

Supplied in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween 20, 50% glycerol, 3 mM DTT.
aqueous glycerol solution

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

S9446-10UG:
S9446-10UG-PW:
S9446-VAR:
S9446-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
051M1568
060M1328
048K1132
106K0549

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Mark E Schnute et al.
The Biochemical journal, 444(1), 79-88 (2012-03-09)
SphK (sphingosine kinase) is the major source of the bioactive lipid and GPCR (G-protein-coupled receptor) agonist S1P (sphingosine 1-phosphate). S1P promotes cell growth, survival and migration, and is a key regulator of lymphocyte trafficking. Inhibition of S1P signalling has been
Zhengshan Zhao et al.
The Journal of biological chemistry, 287(8), 5562-5573 (2011-12-24)
Loss of insulin-producing β-cell mass is a hallmark of type 2 diabetes in humans and diabetic db/db mice. Pancreatic β-cells can modulate their mass in response to a variety of physiological and pathophysiological cues. There are currently few effective therapeutic
Keith S Mascall et al.
Journal of cell science, 125(Pt 9), 2267-2275 (2012-02-22)
Following myocardial infarction, angiogenesis occurs as a result of thrombus formation, which permits reperfusion of damaged myocardium. Sphingosine 1-phosphate (S1P) is a naturally occurring lipid mediator released from platelets and is found in high concentrations at sites of thrombosis. S1P

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