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S5933

Sigma-Aldrich

Anti-STAT3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD01092492
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 92 kDa

species reactivity

human, rat, mouse

technique(s)

immunocytochemistry: 10 μg/mL using A431 cells
immunoprecipitation (IP): 4 μg using STAT3 from 0.5–1 mg of an EGF stimulated human A431 cell lysate
western blot (chemiluminescent): 0.5-2 μg/mL using lysates from EGF stimulated human A431, mouse WEHI and rat L6 cells

UniProt accession no.

P40763

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... STAT3(6774)
mouse ... Stat3(20848)
rat ... Stat3(25125)

General description

Signal transducer and activator of transcription 3 (STAT3) protein is a member of the STAT family of proteins. It consists of a DNA binding domain, a Src homology-2 (SH-2) domain, an N-terminal coiled-coiled domain, and a C-terminal transactivation domain. The STAT3 gene is located on the human chromosome at 17q21.2.

Specificity

The antibody is specific for STAT3. It may detect an additional 66 kDa band using high concentrations or long exposures. The sequence recognized by the antibody (688-722) is identical in mouse and rat STAT3. The first 28 amino acids are identical to mouse STAT3B.

Immunogen

GST fusion protein corresponding to amino acids 688-722 of human STAT3, expressed in bacteria.

Application

Anti-STAT3 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Signal transducer and activator of transcription 3 (STAT3) signaling participates in cancer inflammation. This protein interacts with nuclear factor-κB (NF-κB). STAT3 regulates the function of several genes that take part in proliferation, apoptosis, metastasis, angiogenesis, immune response, and cell differentiation. Overexpression of the STAT3 gene is associated with lymphomas, prostate carcinoma, breast cancer, hepatocellular carcinoma, pancreatic adenocarcinoma, cholangiocarcinoma, and ovarian carcinoma.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

S5933-200UG-PW:
S5933-.1MG:
S5933-200UG:
S5933-VAR:
S5933-BULK:

Certificates of Analysis (COA)

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Hui Jin et al.
Stem cell research, 41, 101586-101586 (2019-11-11)
Autosomal dominant Hyper IgE syndrome (AD-HIES), a rare immune deficiency affecting fewer than one per million people, is caused by heterozygous deleterious mutations in STAT3. STAT3 signaling plays crucial roles in basic cellular functions affecting broad aspects of cellular homeostasis.
Paul A Johnston et al.
Molecular interventions, 11(1), 18-26 (2011-03-29)
Multiple lines of evidence place STAT3 at a central node in the development, progression, and maintenance of many human tumors, and STAT3 has been validated as an anti-cancer target in several contexts. STAT3 modulates the transcription of a variety of
Chien-Hung Lin et al.
Cancers, 12(4) (2020-04-25)
Short noncoding endogenous RNAs, including microRNAs (miRNAs), are associated with the development and metastasis of multiple cancers. Epigallocatechin gallate (EGCG), the most active and abundant polyphenol in green tea, plays a crucial role in the modulation of miRNA expression, which
Ou Li et al.
Medical science monitor : international medical journal of experimental and clinical research, 24, 8510-8523 (2018-11-26)
BACKGROUND Long non-coding RNAs (lncRNAs) have been implicated in various human cancer types. However, the underlying mechanisms involved in hepatocellular carcinoma (HCC) progression remain poorly understood. MATERIAL AND METHODS In this study, lncRNA array was used to identify HCC related
Shigeo Saito et al.
Methods in molecular biology (Clifton, N.J.), 329, 59-79 (2006-07-19)
We describe here the isolation and maintenance of pluripotent embryonic stem (ES) cells from equine blastocysts that have been frozen and thawed. Equine ES cells appear to maintain a normal diploid karyotype in culture. These cells express markers that are
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