S4776

Somatotropin from human pituitary

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), lyophilized powder

• Synonym(s): Growth Hormone, STH
• MDL number: MFCD00081960
• UNSPSC Code: 51111800
• NACRES: NA.32
• biological source: human
• form: lyophilized powder
• Assay: ≥95% (SDS-PAGE)

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• sterility: non-sterile
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.025-0.1 ng/mL cell proliferation using Nb2-11 cells per bioactivity ED₅₀
• mol wt: predicted mol wt ~22 kDa
• drug control: Home Office Schedule 4.2
• impurities: endotoxin, tested
• solubility: PBS: 0.100 mL, clear, colorless (with 0.1% BSA )
• UniProt accession no.: P01241; P10912
• shipped in: ambient
• storage temp.: −20°C
• Gene Information: human ... GH1(2688) , GHR(2690)

Description

General description

Somatotropin or growth hormone is a peptide hormone that is secreted by the anterior pituitary gland. It is essential for growth. The hormone increases muscle mass, reduces fat mass, helps in maintaining normal blood glucose levels and also promotes a balanced lipid profile.

Application

Somatotropin or growth hormone has been used to study the molecular interactions of ephrin type-A receptor 4 precursor (EphA4) with the growth hormone receptor. It has also been used to induce differentiation in mammary epithelial cell line HC11.

Biochem/physiol Actions

Increases mass of most tissues by increasing cell number rather than cell size, mobilizes fat stores.

Physical form

Lyophilized from a 0.2 μm filtered solution in TBS (20 mM Tris, 0.15 M NaCl, pH 8.5) containing 50 μg of BSA per 1 μg of cytokine.

Reconstitution

It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 μg/mL.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable