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Safety & Regulatory

RAB0352

Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr²⁰²/pTyr²⁰⁴), phospho-JNK (pThr¹⁸³/pTyr¹⁸⁵), and phospho-p38 MAPK (pThr¹⁸⁰/pTyr¹⁸²) in cultured cell lines

Name: Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines
Brand: SIGMA

192 assays

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About This Item

UNSPSC Code:

41116158

NACRES:

NA.32

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RAB0348

Phospho-Erk1 (pThr²⁰² / pTyr²⁰⁴) + Erk2(pTyr^185/187) ELISA Kit

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RAB0350

Cell-Based ELISA for detecting phospho-ERK1 / 2 (pThr²⁰² / pTyr²⁰⁴) in cultured cell lines

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RAB0342

Cell-Based ELISA for detecting phospho-P38 MAPK (pThr¹⁸⁰ / pTyr¹⁸²) in cultured cell lines

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RAB0349

Phospho-Erk1 (pThr²⁰² / pTyr²⁰⁴ + Erk2 (pTyr^185/187) and pan-Erk1 / 2 ELISA Kit

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RAB0353

Cell-Based ELISA for detecting phospho-JNK (pThr¹⁸³ / pTyr¹⁸⁵) in cultured cell lines

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Penicillin-Streptomycin

usage

192 assays

species reactivity

mouse, human, rat

technique(s)

ELISA: suitable
capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MAPK1(5594) , MAPK14(1432) , MAPK3(5595) , MAPK8(5599)
mouse ... MAPK1(26413) , MAPK14(26416) , MAPK3(26417) , MAPK8(26419)
rat ... MAPK1(116590) , MAPK14(81649) , MAPK3(50689) , MAPK8(116554)

General description

The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr²⁰² / pTyr²⁰⁴), JNK (pThr¹⁸³ / pTyr¹⁸⁵) and p38 MAPK (pThr^180m / pTyr¹⁸²) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

ERK1/2 (pThr²⁰² / pTyr²⁰⁴), JNK (pThr¹⁸³ / pTyr¹⁸⁵), p38 MAPK (pThr¹⁸⁰ / pTyr¹⁸²) synthetic phosphopeptides

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.

Description

SDS

RABBLOCK5x Blocking Solution (Item F)SDS

RABFIX1Fixing Solution (Item D)SDS

RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS

RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS

RABPLATE1Uncoated MicroplateSDS

RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS

RABSTOP1Stop SolutionSDS

RABTMB1TMB Substrate Reagent (Item J)SDS

RABWASH120X Wash Buffer Concentrate ASDS

RABWASH220X Wash Buffer Concentrate BSDS

See All (10)

Pictograms

GHS08,GHS05,GHS07

Signal Word

Danger

Hazard Statements

H290,H302 + H332,H315,H317,H318,H335,H341,H350,H412

Precautionary Statements

P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.