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Key Documents

Safety Information

R9001

Sigma-Aldrich

Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)

Type X, lyophilized powder

Synonym(s):

Transfer RNA, tRNA

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About This Item

CAS Number:
9014-25-9
MDL number:
MFCD00132200
UNSPSC Code:
41106305
NACRES:
NA.55

grade

for molecular biology

type

Type X

form

lyophilized powder

storage temp.

−20°C

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General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.

Components

tRNA is provided as a lyophilized powder.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

R9001-BULK:
R9001-VAR:
R9001-10KU:
R9001-2KU:
R9001-500UN:
R9001-100UN:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mammalian in vitro splicing assays.
A Mayeda et al.
Methods in molecular biology (Clifton, N.J.), 118, 315-321 (1999-11-05)
Sean P Ryder et al.
Methods in molecular biology (Clifton, N.J.), 488, 99-115 (2008-11-05)
The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to resolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to
Christophe Maris et al.
Nucleic acids research, 48(8), 4521-4537 (2020-03-15)
The polypyrimidine tract binding protein (PTB) is a multi-domain protein involved in alternative splicing, mRNA localization, stabilization, polyadenylation and translation initiation from internal ribosome entry sites (IRES). In this latter process, PTB promotes viral translation by interacting extensively with complex
A simplified method for combined immunohistochemistry and in-situ hybridization in fresh-frozen, cryocut mouse brain sections.
Newton, S.S., et al.
Brain Res. Protoc., 9, 214-214 (2002)
Crystal E Fagan et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9716-9721 (2013-05-01)
After four decades of research aimed at understanding tRNA selection on the ribosome, the mechanism by which ribosomal ambiguity (ram) mutations promote miscoding remains unclear. Here, we present two X-ray crystal structures of the Thermus thermophilus 70S ribosome containing 16S
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