Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

R840-05A

Sigma-Aldrich

Rat Brain Microvascular Endothelial Cells: RBMVEC, adult

Synonym(s):

RBMVEC cells

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

rat brain (Sprague Dawley, adult)

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

morphology

Endothelial

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.


RBMVEC from Cell Applications, Inc. provide an excellent model system to study many aspects of endothelial function and disease, especially those related to the blood-brain barrier (BBB), including interactions between neurons, astorcytes and endothelial cells, brain cognitive function, search for therapeutic modulators, and develop novel drug delivery methods for crossing the BBB.

RBMVEC from Cell Applications, Inc. have been utilized in a number of research publications, for example to:

  • Serve as a gold standard control for endothelial markers VE-cadherin and CD31 expression (Johansson, 2009)
  • Demonstrate a better brain penetration of cardiovascular and anti-stroke drugs Tanshinones IIA and IIB and Cryptotanshinone in the presence of PgP or MRP1/2 inhibitors (Chen, 2007; Yu, 2007; Zhi-Wei, 2007) or by using PEGylated gold nanoparticles (Etame, 2011)
  • Show that removal of excess glutamate from the blood by glutamate oxaloacetate transaminase correlates to a decrease in brain glutamate levels and confers neuroprotection against ischemic stroke (Campos, 2011)
  • Assess, along with Rat Astrocytes (RA) also obtained from Cell Applications, Inc., neuroprotective capabilities of bioenergy stabilizers in an in vitro model of stroke (Liu, 2013)
  • Show that bile acids cause activation of Rac1 and phosphorylation of occluding, explaining increased permeability of the blood brain barrier seen during obstructive cholestasis (Quinn, 2014)
  • Demonstrate that saquinavir-associated dementia in HIV-positive patients is exacerbated by smoking due to the BBB disruptive effects of both nicotine and saquinavir, mediated by decrease in Notch-4 expression and increase in ROS (Manda, 2010)
  • Show that treatment with connexin43 mimetic peptide which transiently blocks gap junction function, reduces vascular leak and can be used to treat of central nervous system ischaemia (Danesh-Meyer, 2012)
  • Demonstrate that oxygen and glucose deprivation increase ROS, cytochrome c levels and caspase-3 activity, induce tight junction disruption and actin stress fiber formation, leading to BBB break down (Alluri, 2013)
  • Investigate the interactions between neurons, astorcytes and endothelial cells by showing that exposure to metabolic stress induces tissue-type plasminogen activator release from neurons which induces AMPK activation, membrane recruitment of GLUT1, and GLUT1-mediated glucose uptake in astrocytes and endothelial cells, followed by the synthesis and release of lactic acid from astrocytes, and that the uptake of this lactic acid via the monocarboxylate transporter-2 promotes survival in neurons (An, 2013)
  • Study the proapoptotic and anti-angiogenic role of p75NTR in choroidal neovascularization (Tahiri, 2013)

Cell Line Origin

Brain

Application

Endothelial vessel function, cell signaling, angiogenesis.

Components

Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO

Quality

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Preparation Note

  • 2nd passage, >500,000 cells in Rat Brain Endothelial Cell Basal Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 16 population

Subculture Routine

Please refer to the RBMVEC Culture Protocol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

R840-05A:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nicholas Sitaras et al.
The American journal of pathology, 185(2), 581-595 (2014-12-06)
Ischemic retinopathies are characterized by sequential vaso-obliteration followed by abnormal intravitreal neovascularization predisposing patients to retinal detachment and blindness. Ischemic retinopathies are associated with robust inflammation that leads to generation of IL-1β, which causes vascular degeneration and impairs retinal revascularization

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service