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Safety Information

R7256

Sigma-Aldrich

RPMI 1640 Vitamins Solution (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

RPMI 1640 Solution, RPMI 1640 Vitamins, Vitamins Solution

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About This Item

UNSPSC Code:
12352205
NACRES:
NA.75

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

yellow to dark yellow

pH

7.2-7.6

shipped in

dry ice

storage temp.

−20°C

General description

Roswell Park Memorial Institute medium is commonly referred as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. RPMI 1640 is used for the serum-free expansion of human lymphoid cells.

Application

RPMI 1640 Vitamins Solution (100×) has been used:
  • in the preparation of CeM1 medium for the isolation of Caenorhabditis elegans germ cells
  • as a component of the amino-acid-deprived medium for the starvation of intestinal porcine enterocytes
  • as a component of the stage-8 medium for culturing of human pluripotent stem cell-derived 3D pancreatic progenitor clusters
RPMI 1640 Vitamins Solution (100×) is used as a supplement in cell culture media.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

CR40333-500ML:
R7256-BULK:
R7256-PH:
R7256-100ML:
R7256-VAR:
R7256PROC:
CR40333-1L:
R7256-500ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Leucine stimulates ASCT2 amino acid transporter expression in porcine jejunal epithelial cell line (IPEC-J2) through PI3K/Akt/mTOR and ERK signaling pathways
Shihai Z, et al.
Amino Acids, 46(12) (2014)
Indranil Biswas et al.
Journal of bacteriology, 189(18), 6521-6531 (2007-07-10)
Streptococcus mutans, the principal causative agent of dental caries, produces four glucan-binding proteins (Gbp) that play major roles in bacterial adherence and pathogenesis. One of these proteins, GbpC, is an important cell surface protein involved in biofilm formation. GbpC is
Simon Kemmerling et al.
Journal of structural biology, 177(1), 128-134 (2011-11-19)
A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the
Simon Kemmerling et al.
Journal of structural biology, 183(3), 467-473 (2013-07-03)
The stochastic nature of biological systems makes the study of individual cells a necessity in systems biology. Yet, handling and disruption of single cells and the analysis of the relatively low concentrations of their protein components still challenges available techniques.
Alexandra S Vagasi et al.
Bio-protocol, 7(15) (2017-09-05)
The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous
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