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Safety Information

R7023

Sigma-Aldrich

Pst I from Providencia stuartii

Restriction Enzyme

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About This Item

CAS Number:
81295-32-1
EC Number:
3.1.21.4 (BRENDA, IUBMB)
MDL number:
MFCD00165594
UNSPSC Code:
12352204

grade

Molecular Biology
for molecular biology

form

buffered aqueous glycerol solution

concentration

10,000 units/mL

shipped in

wet ice

storage temp.

−20°C

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Specificity

Recognition sequence: 5′-CTGCA/G-3′
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Application

PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Comment: Pst I requires optimal reaction conditions in order to avoid star activity.

Physical form

Solution in 10 mM Tris-HCl, pH 7.0, 1 mM EDTA, 250 mM NaCl, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4 °C

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

JAN Code

R7023-BULK:
R7023-500UN:4548173319421
R7023-VAR:
R7023-100UN:4548173319414

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Certificates of Analysis (COA)

Lot/Batch Number
SLBN0525V
SLBL8959V
SLBK8116V
SLBH5596V
SLBH5596

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The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.
N L Brown et al.
FEBS letters, 65(3), 284-287 (1976-06-15)
E Müller et al.
Pflugers Archiv : European journal of physiology, 435(5), 705-712 (1998-04-16)
The influence of diuresis and antidiuresis on the expression of heat shock proteins (HSP) 25, 60, 72 and 73 in the renal cortex and outer and inner medulla of Wistar rats was analysed. Medullary osmolality was reduced by long-term diuresis
T B Britschgi et al.
Applied and environmental microbiology, 57(6), 1707-1713 (1991-06-01)
The identification of the prokaryotic species which constitute marine bacterioplankton communities has been a long-standing problem in marine microbiology. To address this question, we used the polymerase chain reaction to construct and analyze a library of 51 small-subunit (16S) rRNA
Thomas Hiesberger et al.
The Journal of clinical investigation, 113(6), 814-825 (2004-04-07)
Hepatocyte nuclear factor-1beta (HNF-1beta) is a Pit-1, Oct-1/2, UNC-86 (POU)/homeodomain-containing transcription factor that regulates tissue-specific gene expression in the liver, kidney, and other organs. Humans with autosomal dominant mutations of HNF-1beta develop maturity-onset diabetes of the young type 5 (MODY5)
D I Smith et al.
Nucleic acids research, 3(2), 343-353 (1976-02-01)
We describe the isolation of a new class 2 restriction endonuclease from Providencia stuartii 164. Using the procedure of osmotic shock treatment, we have partially purified this enzyme (Pst 1) and have begun preliminary work to characterize its specificity and
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