R7023

Pst I from Providencia stuartii

Restriction Enzyme

• CAS Number: 81295-32-1
• Enzyme Commission number: 3.1.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00165594
• UNSPSC Code: 12352204

Properties

• grade: for molecular biology
• form: buffered aqueous glycerol solution
• concentration: 10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

Specificity

Recognition sequence: 5′-CTGCA/G-3′
Cutting results: a 2-10-fold Pst I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: Inactivated at 80 °C for 20 minutes.

Application

PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SH (B3657).

Comment: Pst I requires optimal reaction conditions in order to avoid star activity.

Physical form

Solution in 10 mM Tris-HCl, pH 7.0, 1 mM EDTA, 250 mM NaCl, 1 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4 °C

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable