R7009
Sty I from Escherichia coli strain carrying pST27
buffered aqueous glycerol solution
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.53
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-C/C(A,T)(T,A)GG-3′
Ligation and recutting results: After 2-10-fold Sty I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Ligation and recutting results: After 2-10-fold Sty I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 15 minutes.
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM KCl, 10 mM 2-mercaptoethanol, 0.1% gelatine (v/v), 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
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