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PROTMEM

Millipore

ProteoPrep® Membrane Extraction Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

General description

ProteoPrep® Membrane Extraction Kit reagents utilize a powerful detergents for higher loading and resolution of proteins in 2D gels. Protein Extraction Reagent Types 3 and 4 are optimized for proteomic studies. Extraction reagents 3 and 4 are ideal for protein extraction prior to isoelectric focusing and 2D electrophoresis. This kit also includes reagents for the reduction and alkylation of disulfide bonds. The ProteoPrep® Membrane Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.

Application

ProteoPrep® Membrane Extraction Kit is designed to prepare highly enriched membrane protein solutions from many types of cells. The final protein solution are suitable for 2D gel electrophoresis.

Features and Benefits

  • Innovative detergent preparations - Improved solubility allows for higher protein loads and greater visibility of low abundance proteins in 2D gels.
  • Two pre-mixed solubilization solutions - Removes interfereing non-membrane proteins prior to extractions, resulting in uncluttered 2D arrays.
  • Pre-measured reducing & alkylating reagents - Easy-to-use reagents provide improved IEF resolution.

Legal Information

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS
  • C0356Protein Extraction Reagent Type 4 23 mLSDS
  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

186.8 °F - closed cup

Flash Point(C)

86 °C - closed cup

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

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PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

キットコンポーネントの情報を参照してください

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ9570
SLCG4273
SLCD1570
SLCB9906
SLCB6162

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Lili Xu et al.
The Journal of general virology, 90(Pt 11), 2695-2703 (2009-07-25)
Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has
Sreepriya Nair et al.
Electrophoresis, 32(24), 3621-3629 (2011-11-22)
Performing 2-DE of lipid-rich multilamellar membranes like myelin is a cumbersome task. However, for understanding its molecular organization and changes during diseases, identification of proteins of myelin is essential. Although the 2-D-proteomic approach of myelin has been employed to understand
Fang Chen et al.
Proteomics, 7(9), 1529-1539 (2007-04-05)
Plant plasma membrane (PM) proteins play important roles in signal transduction during defense response to an attacking pathogen. By using an improved method of PM protein preparation and PM-bound green fluorescent protein fusion protein as a visible marker, we conducted
Carla Cacciotto et al.
BMC microbiology, 10, 225-225 (2010-08-27)
Mycoplasmas are the simplest bacteria capable of autonomous replication. Their evolution proceeded from gram-positive bacteria, with the loss of many biosynthetic pathways and of the cell wall. In this work, the liposoluble protein complement of Mycoplasma agalactiae, a minimal bacterial
Haiqing Sheng et al.
Applied and environmental microbiology, 74(16), 5015-5022 (2008-06-17)
Escherichia coli O157:H7 causes hemorrhagic colitis and the life-threatening hemolytic-uremic syndrome in humans and transiently colonizes healthy cattle at the terminal rectal mucosa. To investigate the role of the O antigen in persistence and colonization in the animal host, we
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