P9120

PNGase F from Elizabethkingia meningoseptica

recombinant, expressed in E. coli, set of 100 units nanomolar unit

• Synonym(s): N-Glycanase^®, N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase
• CAS Number: 83534-39-8
• Enzyme Commission number: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD00131222
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• conjugate: (N-linked)
• specific activity: ≥10 units/mg protein
• mol wt: 36 kDa
• packaging: set of 100 units nanomolar unit
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation

• of recombinant soybean agglutinin (rSBA) in Nicotiana benthamiana (NbrSBA) and Solanum tuberosum (StrSBA)
• of frontal cortical lysate to verify the glycosylation profile of β-secretase (BACE proteins)
• of cell lysate for evaluating the siRNA silencing of cellular prion protein (PrP^c) post transfection

PNGase F is a glycosylasparaginase used to deglycosylate proteins. It is widely used in structure-function studies of glycoproteins.

Used to deglycosylate protein.

Biochem/physiol Actions

PNGase F cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-linked core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F.

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Packaging

Each set includes enzyme, two formulations of 5× reaction buffer (for routine and mass spectrometry downstream analysis), detergent and denaturation solutions

Unit Definition

One unit will catalyze the release of N-linked oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Legal Information

N-Glycanase is a registered trademark of Agilent Technologies Inc

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H311,H315,H317,H318,H334,H361d,H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable