Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

P8045

Sigma-Aldrich

Protein G-Biotin from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

Synonym(s):

Biotinylated Protein G, Protein G Biotin, Protein G-Biotin

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00132111
UNSPSC Code:
12352203
NACRES:
NA.46

recombinant

expressed in unspecified host

Quality Level

200

conjugate

biotin conjugate

form

lyophilized powder

composition

Protein, ~90% biuret

extent of labeling

2-4 mol d-biotin per mol protein

technique(s)

direct ELISA: 1:70,000 using human IgG

storage temp.

2-8°C

General description

Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.

Specificity

Binds IgG only from most mammalian species except cat; binds chicken IgG.

Application

Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.

Packaging

Package size based on protein content

Other Notes

Truncated - lacks albumin, Fab and membrane binding sites.

Preparation Note

Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P8045-BULK-N:
P8045-PH:
P8045-BULK:
P8045-1MG-PW:
P8045-250UG:
P7693-100MG-PW:
P8045-VAR:
P8045-1MG:
P8045PROC:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Electrochemical biosensing based on universal affinity biocomposite platforms
Zacco E, et al.
Biosensors And Bioelectronics, 21(7), 1291-1301 (2006)
Ayumi Taniguchi et al.
Journal of nutritional science and vitaminology, 53(6), 457-463 (2008-01-19)
It is well known that biotin deficiency causes morphological anomalies in hatchlings of fowl. An abundance of biotin in the yolk, therefore, is greatly required for maintaining reproductive functions. Although there is growing evidence for the molecular significance of the
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems: Biosensors, Recognition Receptors, and Microsystem, 21(7), 360-360 (2008)
Pierre-Henri Puech et al.
PloS one, 6(7), e22344-e22344 (2011-07-30)
The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends
Alina Sultanova et al.
Microbiology spectrum, 10(3), e0236921-e0236921 (2022-05-24)
Human herpesvirus-6 (HHV-6) contains two genes (U12 and U51) that encode putative homologues of human G-protein-coupled receptors like CCR1, CCR3, and CCR5. It has been shown that these viral proteins can be expressed on the surface of epithelial and some
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service