Skip to Content
Merck
All Photos(1)

Documents

Safety Information

P7899

Sigma-Aldrich

Anti-Peroxidase antibody produced in rabbit

fractionated antiserum, lyophilized powder

Synonym(s):

Rabbit Anti HRP

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00162388
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

horseradish

technique(s)

indirect ELISA: 1:100,000-1:200,000

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.

Immunogen

Purified peroxidase from horseradish

Application

Anti-Peroxidase antibody produced in rabbit has been used in:
  • immunoblot assays
  • enzyme-linked-immunosorbent assay (ELISA)
  • immunoprecipitation

Biochem/physiol Actions

Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

Reconstitution

Reconstitute with deionized water.

Analysis Note

Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P7899-VAR:
P7899-1ML:
P7899-2ML-PW:
P7899-1ML-PW:
P7899-2ML:
P7899-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Peroxidase from horseradish Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

Sigma-Aldrich

P8375

Peroxidase from horseradish

Rudi Loesel et al.
Journal of neuroscience methods, 155(2), 202-206 (2006-02-17)
Being able to discriminate between neurons and non-neuronal cells such as glia and tracheal cells has been a major problem in insect neuroscience, because glia-specific antisera are available for only a small number of species such as Drosophila melanogaster and
Tyrosine nitration of cytosolic peroxidase is probably triggered as a long distance signaling response in sunflower seedling cotyledons subjected to salt stress
Jain P and Bhatla SC
PLoS ONE, 13(5), e0197132-e0197132 (2018)
Bin Yang et al.
Oncology letters, 15(4), 4662-4668 (2018-03-16)
The present study aimed to assess whether different anesthesia methods (general anesthesia and general anesthesia combined with epidural block) were associated with tumor metastasis during the perioperative period and the possible molecular mechanisms of tumor metastasis. A rat hepatoma tumor
Chilling injury and peroxidase activity changes in ?Fortune? mandarin fruit during low temperature storage
El-HF, et al.
Bulgarian Journal of Plant Physiology, 29(1-2), 44-54 (2003)
Mary Lynn Dear et al.
Development (Cambridge, England), 143(1), 75-87 (2015-11-26)
Synaptogenesis requires orchestrated intercellular communication between synaptic partners, with trans-synaptic signals necessarily traversing the extracellular synaptomatrix separating presynaptic and postsynaptic cells. Extracellular matrix metalloproteinases (Mmps) regulated by secreted tissue inhibitors of metalloproteinases (Timps), cleave secreted and membrane-associated targets to sculpt
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service