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P4937

Sigma-Aldrich

Percoll®

pH 8.5-9.5 (25 °C), suitable for cell culture

Synonym(s):

Density gradient medium

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About This Item

MDL number:
MFCD00131830
UNSPSC Code:
12161902
NACRES:
NA.75

sterility

aseptically filled

Quality Level

200

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

pH

8.5-9.5 (25 °C)

density

1.13 g/mL±0.005 g/mL at 25 °C (lit.)

storage temp.

2-8°C

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General description

Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials. Due to its heterogeneity in particle size, sedimentation occurs at different rates, spontaneously creating very smooth, isometric gradients in the range of 1.0-1.3 g/ml.

Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll® has been used in the centrifugal separation of:
  • neonatal rat ventricular cardiomyocytes
  • leucocytes from a blood sample
  • peritubular myoid (PM) cells
Suitable for use in preparation of single cell suspension for sequencing.

Features and Benefits

  • Low osmolality permits precise adjustment to physiological conditions without significant interference from the medium.
  • Compatible with living cells and viruses and allows separation and recovery of intact, fully active systems.
  • Impermeable to biological membranes, resulting in no change of buoyant density of particles during centrifugation.
  • Spontaneous formation of gradient during centrifugation allows mixing of large sample volumes in the centrifuge tubes.
  • Low viscosity results in rapid formation of gradients and particle separation.
  • Most biological particles having sedimentation coefficient values greater than 60S can be successfully isolated in Percoll gradients.

Legal Information

Percoll is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

P4937-500ML:4548173963662
P4937-100ML:4548173963648
P4937-PPG:
P4937PROC:
P4937-PH:
P4937-VAR:
P4937-100ML-KC:
P4937-25ML-PW:
P4937-500ML-PW:
P4937-100ML-PW:
P4937-BULK:
P4937-25ML:4548173963655

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).
H Pertoft et al.
Analytical biochemistry, 88(1), 271-282 (1978-07-15)
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Yanping P Chen et al.
The Journal of eukaryotic microbiology, 56(2), 142-147 (2009-05-22)
Nosema ceranae, a microsporidian parasite originally described from Apis cerana, has been found to infect Apis melllifera and is highly pathogenic to its new host. In the present study, data on the ultrastructure of N. ceranae, presence of N. ceranae-specific
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