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Safety Information

P2143

Sigma-Aldrich

Protease from Aspergillus saitoi

Type XIII, ≥0.6 unit/mg solid

Synonym(s):

Molsin

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About This Item

CAS Number:
9025-49-4
Enzyme Commission number:
3.4.23.18 (BRENDA, IUBMB)
EC Number:
232-796-2
MDL number:
MFCD00132092
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Aspergillus sp. (Aspergillus saitoi)

Quality Level

200

type

Type XIII

form

solid

specific activity

≥0.6 unit/mg solid

foreign activity

alkaline protease, essentially free

storage temp.

−20°C

Application

Protease from Aspergillus saitoi has been used in a study to assess sequence coverage and resolution in hydrogen exchange of large proteins. It has also been used in a study to investigate the conversion of soybean curd isoflavone glycosides to their aglycones through β-glucosidase..

Biochem/physiol Actions

Protease from Aspergillus saitoi was shown to also function as a β-glucosidase.

Unit Definition

One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 2.8 at 37 °C (color by Folin-Ciocalteu reagent).

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Precautionary Statements

P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

P2143-5G:
P2143-VAR:
P2143-100G:
P2143-25G:
P2143-BULK:

Certificates of Analysis (COA)

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F J Moralejo et al.
Applied microbiology and biotechnology, 54(6), 772-777 (2001-01-11)
A gene encoding the sweet-tasting protein thaumatin (tha) with optimized codon usage was expressed in Aspergillus awamori. Mutants of A. awamori with reduced proteolytic activity were isolated. One of these mutants, named lpr66, contained an insertion of about 200 bp
T M A Gronewold et al.
Biosensors & bioelectronics, 22(9-10), 2360-2365 (2006-11-03)
Degradation of a crude protein mixture by proteases with pH optima from acidic to basic was followed in real time using a surface acoustic wave biosensor in Love-wave geometry. Proteases EC 3.4.23.18 from Aspergillus saitoi, EC 3.4.21.62 from Bacillus licheniformis
E Ichishima et al.
The Biochemical journal, 339 ( Pt 3), 589-597 (1999-04-24)
For the construction of an overexpression system of the intracellular 1,2-alpha-mannosidase (EC 3.2.1.113) gene (msdS) from Aspergillus saitoi (now designated Aspergillus phoenicis), the N-terminal signal sequence of the gene was replaced with that of the aspergillopepsin I (EC 3.4.23.18) gene
Laetitia Cravello et al.
Rapid communications in mass spectrometry : RCM, 17(21), 2387-2393 (2003-10-31)
The combination of hydrogen exchange and mass spectrometry has been widely used in structural biology, providing views on protein structure and protein dynamics. One of the constraints is to use proteases working at low pH and low temperature to limit
I E Mattern et al.
Molecular & general genetics : MGG, 234(2), 332-336 (1992-08-01)
In the present study, the extracellular protease activity in a strain of the filamentous fungus Aspergillus niger was investigated and mutant strains deficient in the production of extracellular proteases were isolated. The major protease, which is responsible for 80-85% of
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