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P1782

Sigma-Aldrich

Protein kinase Cα isozyme human

≥70% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

Synonym(s):

PKCα

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About This Item

MDL number:
MFCD00241710
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in baculovirus infected insect cells

Assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥250 units/μg protein

mol wt

80-81 kDa by SDS-PAGE

UniProt accession no.

P17252

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKCA(5578)

Biochem/physiol Actions

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

Unit Definition

One unit will transfer 1 pmol of phosphate from ATP to Histone H1 in 1 minute at pH 7.4 at 30 °C

Physical form

Supplied as a buffered aqueous glycerol solution, containing Trizma buffer, NaCl, DTT and Glutathione.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Cartagena Act

Cartagena Act Listed

JAN Code

P1782-VAR:
P1782-BULK:
P1782-5UG:
P1782-20UG:

Certificates of Analysis (COA)

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Heidi Budde et al.
Antioxidants (Basel, Switzerland), 10(7) (2021-08-07)
Oxidative stress is defined as an imbalance between the antioxidant defense system and the production of reactive oxygen species (ROS). At low levels, ROS are involved in the regulation of redox signaling for cell protection. However, upon chronical increase in

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