O3514
Osteopontin from bovine milk
lyophilized powder
Synonym(s):
Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
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About This Item
Recommended Products
biological source
bovine milk
Quality Level
sterility
sterile
form
lyophilized powder
mol wt
~60 kDa
packaging
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
storage temp.
−20°C
Gene Information
cow ... SPP1(281499)
Related Categories
General description
Osteopontin (OPN) is a multifunctional protein. It is released by macrophages, T cells and epithelial cells. It has 261 amino acid residues with a molecular mass of 29,283 Da. Osteopontin is also called as uropontin. Osteopontin from bovine milk is highly glycosylated and phosphorylated. SPP1 gene (secreted phosphoprotein 1) codes for osteopontin. This gene is located on human chromosome 4 and cow chromosome 6. It has seven exons and six introns.
Biochem/physiol Actions
A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity.
Osteopontin stimulates cell mediated responses, chemotaxis of inflammatory cells and anti-inflammatory responses. It binds to av-containing integrins and stimulate cell adhesion and migration. Osteopontin promotes Th1 type immunity.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to mediate 293 cell adhesion.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Milk and Milk Products in Human Nutrition (2011)
Milk Proteins: From Expression to Food (2014)
Molecular analysis of the Bacillus subtilis bacteriophage SPP1 region encompassing genes 1 to 6: The products of gene 1 and gene 2 are required for pac cleavage.
Chai S, et al.
Journal of Molecular Biology, 224(1), 87-102 (1992)
Guidebook to the Extracellular Matrix, Anchor, and Adhesion Proteins (1999)
D D Hu et al.
The Journal of biological chemistry, 270(44), 26232-26238 (1995-11-03)
Osteopontin (OPN) is an extracellular matrix protein that binds to integrin alpha v beta 3. Here we demonstrate that two other integrins, alpha v beta 1 and alpha v beta 5, are also receptors for OPN. Human embryonic kidney 293
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