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N6786

Sigma-Aldrich

Anti-Notch1 antibody, Mouse monoclonal

clone mN1A, purified from hybridoma cell culture

Synonym(s):

Notch antibody

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About This Item

MDL number:
MFCD03455659
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

mN1A, monoclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using extracts of cells stably transfected with a truncated Notch1 (32D-mN1-ICDOP that expresses the intracellular mouse Notch1 molecule-65 kDa).

isotype

IgG1

UniProt accession no.

P46531

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOTCH1(4851)
mouse ... Notch1(18128)

General description

Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.
The Notch protein family is a group of highly conserved proteins important in the determination of cell fate and maintenance of progenitors in many developmental systems.In mammals, four Notch genes were identified (Notch1-4) in a wide variety of cells and play a crucial role in differentiation and development. Notch proteins contain three main domains: an extracellular domain containing tandem epidermal growth factor repeats, and three lin-12/Notch repeats, a transmembrane domain, and an intracellular domainthat contains six cdc1-/SW16/ankyrin repeats, nuclear localization signals, and a C-terminal OPA/PEST region.

Specificity

Recognizes human and mouse Notch1 (~300 kDa and 120 kDa) once Notch1 is cleaved. The antibody does not recognize Notch 2, 3, or 4. It has high affinity for the activated intracellular form of Notch.

Immunogen

recombinant protein containing the cdc10-NCR region of mouse Notch1.

Application

Anti-Notch1 antibody, mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • immunohistochemistry

Biochem/physiol Actions

NOTCH is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism. Fibronectin functions both as a regulator of cellular processes and as an important scaffolding protein to maintain and direct tissue organization and ECM composition.Fibronectin is suggested to enhance cell adhesion, spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

N6786-VAR:
N6786-200UL:
N6786-BULK:

Certificates of Analysis (COA)

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Annapurna Pranatharthi et al.
Journal of experimental & clinical cancer research : CR, 38(1), 392-392 (2019-09-07)
Radioresistance remains a challenge to the successful treatment of various tumors. Intrinsic factors like alterations in signaling pathways regulate response to radiation. RhoC, which has been shown to modulate several tumor phenotypes has been investigated in this report for its
Type II endometrial cancer overexpresses NILCO: A preliminary evaluation
Daley-Brown D, et al.
Disease Markers, 2017 (2017)
Monica Battle et al.
International journal of cancer, 134(7), 1605-1616 (2013-10-12)
To investigate whether obesity induces a leptin-Notch signaling axis in breast cancer (BC), leptin-induced Notch was determined in human MCF-7 and MDA-MB231 and mouse E0771 cells and in E0771-BC hosted by syngeneic lean and diet-induced obesity (DIO) C57BL/6J female mice.
L L Song et al.
Oncogene, 27(44), 5833-5844 (2008-06-19)
Notch-1 inhibits apoptosis in some transformed cells through incompletely understood mechanisms. Notch-1 can increase nuclear factor-kappa B (NF-kappaB) activity through a variety of mechanisms. Overexpression of cleaved Notch-1 in T-cell acute lymphoblastic leukemia cells activates NF-kappaB via interaction with the
L A Milner et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(23), 13014-13019 (1996-11-12)
Effective hematopoiesis requires the commitment of pluripotent and multipotent stem cells to distinct differentiation pathways, proliferation and maturation of cells in the various lineages, and preservation of pluripotent progenitors to provide continuous renewal of mature blood cells. While the importance
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