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Safety Information

N6530

Sigma-Aldrich

N1 Medium Supplement (100×)

solution, sterile-filtered, suitable for cell culture

Synonym(s):

N1 Supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.77

sterility

sterile-filtered

Quality Level

300

form

solution

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Related Categories

General description

N1 Medium Supplement (100×) can be used as a supplement to produce complete N1 neural cell growth media or closely related media.

Application

N1 Medium Supplement (100×) has been used as:
  • a component of the proliferation medium for culturing oligodendrocyte-like cells and neuroblastoma cells
  • a supplement in the Dulbecco′s modified Eagle medium (DMEM/F12) to culture human oligodendrocytes
  • a component of the α-minimum essential medium (MEM) for culturing induced primary retinal pigment epithelium (ipRPE) monolayers

Other Notes

Contains 0.5mg/ml recombinant human insulin, 0.5mg/ml human transferrin (partially iron-saturated), 0.5μg/ml sodium selenite, 1.6mg/ml putrescine, and 0.73μg/ml progesterone.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

N6530-VAR:
N6530PROC:
N6530-BULK:
N6530-PH:
N6530-5ML:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ludmila A Voloboueva et al.
Investigative ophthalmology & visual science, 46(11), 4302-4310 (2005-10-27)
To determine whether (R)-alpha-lipoic acid (LA) protects cultured human fetal retinal pigment epithelial (hfRPE) cells against oxidative injury and identify the pathways that may mediate protection. Cultured hfRPE cells were pretreated with various concentrations of LA for 14 to 16
Miguel Flores-Bellver et al.
Journal of extracellular vesicles, 10(13), e12165-e12165 (2021-11-10)
Age-related macular degeneration (AMD) is a leading cause of blindness worldwide. Drusen are key contributors to the etiology of AMD and the ability to modulate drusen biogenesis could lead to therapeutic strategies to slow or halt AMD progression. The mechanisms
Michael Craigie et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 13(2), 126-142 (2017-11-22)
JC virus (JCV) is a human polyomavirus and the etiologic agent of the demyelinating disease progressive multifocal leukoencephalopathy (PML). PML is observed in patients with underlying immunocompromising conditions, suggesting that neuro-immune interactions between peripheral immune cells and neuro-glia play an
Tomasz M Stępkowski et al.
Cellular and molecular neurobiology, 37(6), 1055-1066 (2016-11-11)
Perturbations in the transport of mitochondria and their quality control in neuronal cells underlie many types of neurological pathologies, whereas systems enabling convenient analysis of mitochondria behavior in cellular models of neurodegenerative diseases are limited. In this study, we present
Lucia Lichvarova et al.
Scientific reports, 9(1), 10603-10603 (2019-07-25)
Forebrain glial cells - ependymal cells and astrocytes -acquire upon injury- a "reactive" phenotype associated with parvalbumin (PV) upregulation. Since free radicals, e.g. reactive oxygen species (ROS) play a role in the pathogenesis of multiple sclerosis, and that PV-upregulation in
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